Protein Info for Echvi_4167 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00266: Aminotran_5
  • amino acids 7 to 367 (361 residues), 285.4 bits, E=1.4e-88
• PF01053: Cys_Met_Meta_PP
  • amino acids 52 to 206 (155 residues), 28.5 bits, E=1.2e-10
• PF00155: Aminotran_1_2
  • amino acids 54 to 281 (228 residues), 34.4 bits, E=3e-12
• PF01041: DegT_DnrJ_EryC1
  • amino acids 72 to 205 (134 residues), 27.7 bits, E=3.2e-10

Best Hits

Swiss-Prot: 47% identical to ISCS_METCA: Cysteine desulfurase IscS (iscS) from Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)

KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 58% identity to gfo:GFO_0447)

MetaCyc: 45% identical to cysteine desulfurase IscS (Escherichia coli K-12 substr. MG1655)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7)" in subsystem Alanine biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (9/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (5/6 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• superpathway of L-alanine biosynthesis (2/4 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• molybdopterin biosynthesis (2/6 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (2/10 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (2/11 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7

Sequence Analysis Tools

See L0G5T5 at UniProt or InterPro

Protein Sequence (385 amino acids)

>Echvi_4167 Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes (Echinicola vietnamensis KMM 6221, DSM 17526)
MNSNTKIYLDYNSTTPCDEKVVEAMLPYFSEKFGNSSSSTHSFGWDAEEAVDIAREQIAE
LINSKSNQLYFTSGATEAINLALIGTCEALKPKGKHIISCTTEHQAVLSTLQELEKRGFN
ITLLKVDGNGNLDLDAFQEAIRPETILTCIMYANNETGIIHPIQKIAEIAHAKGVLVMSD
ITQAVGKIPLDIEGSGIDIATFSAHKIYGPKGIGALYIRNRTLLNISPIHFGGGQERGLR
PGTLNVPSIVGFGMACQLCIESMSQESQRLKKLRDKIEQELLTLGDIKINSFNEHRLPHM
SNITFKNIEGNKLLRLMGNLAISQGSACSSGTIEPSHVLLSYGHSKEAALSSIRICLGRQ
TTSQEVEIAIKTIKSTIKNLRKVLI