Protein Info for Echvi_3727 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Phosphoenolpyruvate carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00311: PEPcase
  • amino acids 74 to 283 (210 residues), 79.9 bits, E=1.1e-26
  • amino acids 304 to 844 (541 residues), 393.3 bits, E=2.3e-121
• PF14010: PEPcase_2
  • amino acids 463 to 714 (252 residues), 30.5 bits, E=1.5e-11

Best Hits

KEGG orthology group: K01595, phosphoenolpyruvate carboxylase [EC: 4.1.1.31] (inferred from 62% identity to mtt:Ftrac_3278)

Predicted SEED Role

"Phosphoenolpyruvate carboxylase (EC 4.1.1.31)" in subsystem Fermentations: Mixed acid or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.31)

MetaCyc Pathways

• CO₂ fixation into oxaloacetate (anaplerotic) (2/2 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• mixed acid fermentation (10/16 steps found)
• C4 photosynthetic carbon assimilation cycle, NAD-ME type (5/11 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (7/14 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.31

Sequence Analysis Tools

See L0G151 at UniProt or InterPro

Protein Sequence (849 amino acids)

>Echvi_3727 Phosphoenolpyruvate carboxylase (Echinicola vietnamensis KMM 6221, DSM 17526)
MSNTYQTEVAKRFTIYNSLFLDLPFDDIYRTGTLLPILSSECKKGFAEGKTPKEIINSFF
EGMLASNSSKEKHNLLFQLVQYVERQVVLFDSIEDAAFEKINDVKGKGTMNALISRVESD
KKKAELIEKLRTFSIRLTLTAHPTQFYPGNVLAIITDLESAIRKDDLGSIDALLRQLGKT
AFINKEKPSPYEEAVSLCWFLEHVFYKSIPDIMARVLRKLDIPLHEWDNPDLLKVGFWPG
GDRDGNPFVTHQITMDVADKLQETILRCLYRDVRKVRRRMTFKGVESLMLEAENGIYKTL
YGGEKVLKSKEDLLKILLKAREIIIESHDGLFLDILDEFILKVNVFGFYFASMDMRQDSR
KHAALWEEIIEVTKGKDALKKYQEGTEKEQIETILSFTELPNPKTLKDEFHQEMLESIAS
ISYVQDNNGSEGLHRYIISNCQSELHILQVYQLNKLTLAPKGDLKLDIVPLFETIDDLAA
APEIMERLYSNPVYGGHLKSRGNKQSIMLGFSDGTKDGGYIRANWSILRAKEELTKASRK
YEVSVVFFDGRGGPPARGGGNMHNFYASLGDQVENEEIQVTIQGQTISANYGKPVSCTYN
LEQLLSAGLENHLYPTEENRLTDDQRALIDEMADISYQAYKEFKSHPQFVSYLEKVTPLK
FFGKTNIGSRPLKRSKGEAMKFEDLRAIPFVGSWAQMKQNIPGFYGVGKAITEMEKRGKK
DQVTKLYQDSLFFRSLMGNSMQSLAKSYYPATAYLKNDKEYGEFWELMHSEYQQSFKKIL
EISGMHELLEDNAVSSQSIAIREKIVLPLITIQQYAIQEMLETGKENTVLQKLILRTMFG
IINAARNAA