Protein Info for Echvi_3385 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase" amino acids 19 to 215 (197 residues), 255.9 bits, see alignment E=2.3e-80 PF00926: DHBP_synthase" amino acids 22 to 214 (193 residues), 287.1 bits, see alignment E=5.6e-90 PF00925: GTP_cyclohydro2" amino acids 227 to 388 (162 residues), 248.4 bits, see alignment E=2.7e-78 TIGR00505: GTP cyclohydrolase II" amino acids 228 to 413 (186 residues), 240.6 bits, see alignment E=9.6e-76

Best Hits

Swiss-Prot: 75% identical to RIBBA_CYTH3: Riboflavin biosynthesis protein RibBA (ribBA) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 78% identity to mtt:Ftrac_0930)

MetaCyc: 44% identical to GTP cyclohydrolase II (Chlamydia trachomatis)
GTP cyclohydrolase II. [EC: 3.5.4.25]

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G455 at UniProt or InterPro

Protein Sequence (422 amino acids)

>Echvi_3385 GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase (Echinicola vietnamensis KMM 6221, DSM 17526)
MHFELSIEEKIMAEEIKLDSIEEAIEAIKNGEVVIVVDDEDRENEGDFVCAAEKVTPEII
NFMATHGRGLICAPIIEDRCEELGLELMVGRNTATYETPFTVSVDLIGHGCTTGISASDR
AKTIKALIDDDIHPDELGKPGHIFPLKAKRGGVLRRTGHTEAAIDLARLAGLYPAGVLVE
IMNDDGTMARVPDLVEVAKRFDLKLVSIKDLIAYRLKNESLIKREIGVEMPTEFGDFDLI
AFRQTNTDEIHMALVKGEWKEGEPILVRVHSSCVTGDIFGSCRCDCGPQLHSAMEMVNQA
GKGVVLYMNQEGRGIGLINKLKAYKLQEEGMDTVQANQALGFPMDKRDYGVGAQILRDLG
VSKIKLLTNNPTKRAGLLGYGLEIVETVALEISPNAHNERYLTTKRDKMGHQILKKEINK
MK