Protein Info for Echvi_3321 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Beta-galactosidase/beta-glucuronidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 820 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 32 to 199 (168 residues), 67.2 bits, see alignment E=4.3e-22 PF00703: Glyco_hydro_2" amino acids 209 to 309 (101 residues), 53.6 bits, see alignment E=8.9e-18 PF02836: Glyco_hydro_2_C" amino acids 314 to 477 (164 residues), 97.7 bits, see alignment E=1.8e-31 PF16355: DUF4982" amino acids 637 to 702 (66 residues), 75.2 bits, see alignment E=7.8e-25 PF18565: Glyco_hydro2_C5" amino acids 715 to 814 (100 residues), 96.1 bits, see alignment E=2.7e-31

Best Hits

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 65% identity to bth:BT_4667)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G059 at UniProt or InterPro

Protein Sequence (820 amino acids)

>Echvi_3321 Beta-galactosidase/beta-glucuronidase (Echinicola vietnamensis KMM 6221, DSM 17526)
MRFRSILICLLLIGSICSFSHAQRVRKSWPLKEDWRFSKGEQEEAYQIDFDDREWESVQV
PHDWAIYGPFDESNDLQIVAVTQNGEKVATKKTGRTGGLPYMGVGWYRRAFEVPGFDPEK
QRVKLIFDGAMSEAQVYVNGEKVGFWPYGYNSFYFDVTDFLHADGTKNQLAVRLENKPQS
SRWYPGAGLYRNVHVLVTPKIHVPVWGTFVTTPYVSEEYASVNIKTELEHVSEGTDISIE
TSILDLSGKVVAKKKNVQRLNHGRPLEQFLTVDHPELWSPESPHLYVAKSVISIEGREID
EFTTRFGIRKIEFIADKGFFLNGQRRKFQGVNLHHDLGPLGAAVNRSAIKRQLQLMKDMG
ADAIRTSHNMPAPELVELCDEMGLMVMVESFDEWDVAKCKNGYHRFFDDWAEKDLVNMVR
HYRNHPSVVMWSIGNEVPTQCSADGYQVAKFLQDICHREDPTRLVTAGMDQISCVLDNGF
AAMIDVTGFNYRTHRYEEGYQKLPHSLVLGSETGSTVSSRGVYHFPVEKGAQVIHENQQS
SAYGLEYCSWSNLPEDDFALADDFDWTLGQFVWTGFDYLGEPTPYDTDAWPNHSSMFGIV
DLAGIPKDRYYLYKSVWKPEEETLHILPHWNWEGREGQKTPLFVYTNYPSAELFINGESQ
GKQTKHKEGTLQERYRLMWMDAIYEPGEVKVVAYDSLGKKAAEKILHTAGKPHHIELVPE
NKRIAVEGDELAFIRVQVVDKEGRVCPTDQRSLSFDVAGAGAFRAVANGDPGSLELFHEP
QMKVFNGQLTLIVSAKEAGNIHIKATAKGLKNGETIMEAY