Protein Info for Echvi_3133 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: DNA topoisomerase I, bacterial

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 773 TIGR01051: DNA topoisomerase I" amino acids 5 to 495 (491 residues), 631.8 bits, see alignment E=9.4e-194 PF01751: Toprim" amino acids 5 to 113 (109 residues), 78.6 bits, see alignment E=4.8e-26 PF01131: Topoisom_bac" amino acids 128 to 494 (367 residues), 365.5 bits, see alignment E=5.8e-113 PF13368: Toprim_C_rpt" amino acids 595 to 650 (56 residues), 41.2 bits, see alignment 1.9e-14 amino acids 656 to 706 (51 residues), 53.8 bits, see alignment 2.2e-18 amino acids 713 to 765 (53 residues), 51 bits, see alignment 1.6e-17

Best Hits

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 71% identity to mtt:Ftrac_3551)

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G3E3 at UniProt or InterPro

Protein Sequence (773 amino acids)

>Echvi_3133 DNA topoisomerase I, bacterial (Echinicola vietnamensis KMM 6221, DSM 17526)
MPKNLVIVESPAKAKTIEGYLGKDYKVTSSYGHVRDLPKGDKAIDIKNHFKPTYEVTSDK
KEVIKQLKKMVKDSDTIYLASDDDREGEAISWHLKEVLKLDNDKTKRIVFREITKNAITK
AIENPRSIDIDLVNAQQARRILDRLVGFELSPVLWKKVKAGLSAGRVQSVAVRFIVERER
EIDKFESKSSYKVTAIFNVKGKELNAELPKKFETQEEAEAFLKNCLEADFSIKNLEKKPA
KKTPAAPFTTSTLQQEASRKLSFSVAQTMTLAQRLYEAGKITYMRTDSVNLSQEAMESAQ
NQIISAFGPEYHKSRKYTSKSEGAQEAHEAIRPTNFANEEISGERNEQRLYELIWKRAIA
SQMADALLEKTLVTIGISNSDLTLSASGEVIKFEGFLKVYLESTDDEEEEENNDNKGLLP
PLTIGQDLDLIEMKSRQSFTRPPARYTEASLVKKLEEMGIGRPSTYAPTISTIQKRNYVI
KESRDGKPRNYTEMIIKDGSFSKAEKTENYGSDKNKLFPTNIAMVVNDFLVDHFPNVIDY
KFTAKVEKEFDDIAHGIQQWDNMIENFYGKFHNTVEEAETVERANVSSSRELGKDPKTGK
PVIARLGKFGPLVQIGDQDDEEKQFASLKKGQFIENITFEDAMELFKLPRDVGMFEDKKI
VAAIGRFGPYIRHDGKFVSLGKEYDPLSVNEEEAIQLIKDKREADAKKHIKSFDENPDIQ
ILNGRWGPYIKFAKKNYKIPKDKVAEDLSYEETIEIIENQPEKKKGRFSKKKK