Protein Info for Echvi_3052 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: 3-deoxy-D-manno-octulosonate cytidylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 269 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details TIGR00466: 3-deoxy-D-manno-octulosonate cytidylyltransferase" amino acids 28 to 258 (231 residues), 209.4 bits, see alignment E=2.7e-66 PF02348: CTP_transf_3" amino acids 31 to 239 (209 residues), 174.5 bits, see alignment E=2.9e-55 PF12804: NTP_transf_3" amino acids 45 to 149 (105 residues), 32.6 bits, see alignment E=9e-12

Best Hits

Swiss-Prot: 54% identical to KDSB_FLAJ1: 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) from Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101)

KEGG orthology group: K00979, 3-deoxy-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase) [EC: 2.7.7.38] (inferred from 55% identity to coc:Coch_0027)

Predicted SEED Role

"3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38)" in subsystem KDO2-Lipid A biosynthesis (EC 2.7.7.38)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.38

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G368 at UniProt or InterPro

Protein Sequence (269 amino acids)

>Echvi_3052 3-deoxy-D-manno-octulosonate cytidylyltransferase (Echinicola vietnamensis KMM 6221, DSM 17526)
MLFSSSIKSAKKHFTGLTSPLFFLKLHMKIVAMIPARYGATRFPGKLTSDLCGKPVIVRT
YLSTMATGLFDKVIVVTDHKQIATQIEQEGGEVFFSQKAHESGSDRIAEAVADVEADVVV
NVQGDEPFQDKESLSGLVNAFTDKSVRVASLMRKIEDDVDVINPNSVKVVVDKNSFALYF
SRCPIPFVRDKFQPDYFRHIGVYAYTKELLMTYTNWEKTMLEKAEMLEQLRLLENGIRIK
MVETTHEAVAIDTKADLDRAIAFYQRNYQ