Protein Info for Echvi_2011 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate reductase (IPP and DMAPP forming)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 298 PF02401: LYTB" amino acids 3 to 288 (286 residues), 278.5 bits, see alignment E=2.7e-87 TIGR00216: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase" amino acids 3 to 289 (287 residues), 223.2 bits, see alignment E=2e-70

Best Hits

Swiss-Prot: 72% identical to ISPH_CYTH3: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) from Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469)

KEGG orthology group: K03527, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [EC: 1.17.1.2] (inferred from 72% identity to chu:CHU_0087)

Predicted SEED Role

"4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2)" in subsystem Isoprenoid Biosynthesis or polyprenyl synthesis (EC 1.17.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.2

Use Curated BLAST to search for 1.17.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FWI0 at UniProt or InterPro

Protein Sequence (298 amino acids)

>Echvi_2011 (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate reductase (IPP and DMAPP forming) (Echinicola vietnamensis KMM 6221, DSM 17526)
MQVTIDKNSGYCFGVEFAIKMAEDEMEDGGRLYCLGDIVHNDMEVKRLSDKGLVVIDRDE
LKELSDCKVLIRAHGEPPETYKLAIENNIELIDASCPVVLKLQHRVKTAFDKIEKKDGQI
VIYGKKGHAEVIGLTGQTMEKAIVVMEDEDLEKIDFSKPVTLFSQTTKSTKGFYALKAKI
EEKMAAANQSLEEIDFTANDSICRQVSNREPQLTRFANENDVIIFVAGKKSSNGRALYQV
CKAQNPRSYFVENEGEIDPDWFANGDKVGICGATSTPMWLMEQVMGYINSLDESITHV