Protein Info for Echvi_2007 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: putative bacillithiol system thiol disulfide oxidoreductase, YpdA family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 326 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF07992: Pyr_redox_2" amino acids 5 to 295 (291 residues), 103.1 bits, see alignment E=9.6e-33 TIGR04018: putative bacillithiol system oxidoreductase, YpdA family" amino acids 5 to 319 (315 residues), 467 bits, see alignment E=1.1e-144 PF13738: Pyr_redox_3" amino acids 7 to 290 (284 residues), 312 bits, see alignment E=2.3e-96 PF13434: Lys_Orn_oxgnase" amino acids 68 to 189 (122 residues), 32.3 bits, see alignment E=3.2e-11

Best Hits

Swiss-Prot: 42% identical to YPDA_BACSU: Uncharacterized protein YpdA (ypdA) from Bacillus subtilis (strain 168)

KEGG orthology group: K00384, thioredoxin reductase (NADPH) [EC: 1.8.1.9] (inferred from 60% identity to lby:Lbys_2762)

Predicted SEED Role

"Thioredoxin reductase (EC 1.8.1.9)" in subsystem Glycine reductase, sarcosine reductase and betaine reductase or Thioredoxin-disulfide reductase or Wyeosine-MimG Biosynthesis (EC 1.8.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.9

Use Curated BLAST to search for 1.8.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G056 at UniProt or InterPro

Protein Sequence (326 amino acids)

>Echvi_2007 putative bacillithiol system thiol disulfide oxidoreductase, YpdA family (Echinicola vietnamensis KMM 6221, DSM 17526)
MKKIDVLIVGAGPIGLACGIEAEKNELDYIILEKGTLVNSIYNYPVNMTFFSTSEKLEMG
GIPFMSVNKQPTRPEALEYYRRIAKHFDLNINLYEGVKSVERQADGAFLVYSEKETYFAE
KVVLSTGFYDIPNKLNVPGEDLAKVTHYYKEPWPYIGQKVIVVGGGNSGVDVALETWRKG
AEVTMVLKEPTVDQNVKYWVLPDIENRIKEGSIGAYFSSSIKEIREQEVVIDTPQGEKTL
ENDFVLAMTGYRPNFELLDQLGVGLTLDEKRRPCFDGNQESNVPGLYLAGVVCGGLNTRE
FFIENTIVHAQAIFTDIMNKRKQLVK