Protein Info for Echvi_2000 in Echinicola vietnamensis KMM 6221, DSM 17526

Updated annotation (from data): aspartate kinase / homoserine dehydrogenase (EC 2.7.2.4; EC 1.1.1.3)
Rationale: Important for fitness in most defined media. 40% identical to a bifunctional aspartokinase/homoserine dehydrogenase from Arabidopsis (O81852).
Original annotation: aspartate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 815 transmembrane" amino acids 463 to 478 (16 residues), see Phobius details PF00696: AA_kinase" amino acids 2 to 282 (281 residues), 163.7 bits, see alignment E=1.9e-51 TIGR00657: aspartate kinase" amino acids 3 to 457 (455 residues), 393.8 bits, see alignment E=6e-122 PF22468: ACT_9" amino acids 316 to 375 (60 residues), 67.7 bits, see alignment 1.8e-22 amino acids 396 to 456 (61 residues), 85 bits, see alignment 7.3e-28 PF13840: ACT_7" amino acids 389 to 454 (66 residues), 32.9 bits, see alignment E=1.4e-11 PF03447: NAD_binding_3" amino acids 470 to 604 (135 residues), 56.5 bits, see alignment E=1.3e-18 PF00742: Homoserine_dh" amino acids 612 to 808 (197 residues), 185.9 bits, see alignment E=1.8e-58

Best Hits

KEGG orthology group: K12524, bifunctional aspartokinase / homoserine dehydrogenase 1 [EC: 1.1.1.3 2.7.2.4] (inferred from 50% identity to dfe:Dfer_3152)

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3, 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FYX9 at UniProt or InterPro

Protein Sequence (815 amino acids)

>Echvi_2000 aspartate kinase / homoserine dehydrogenase (EC 2.7.2.4; EC 1.1.1.3) (Echinicola vietnamensis KMM 6221, DSM 17526)
MKIIKFGGSSIANYENIQKVFSIIEQKSEKEAFALVFSAFGGVTEQLLQCANIAQQSEES
YHTILQELEKRHLEIVKKLVPVQQQSTALTFVKVRFNELGDLFHGIYLIKECSNRTMDYV
LSFGERLSNFILAAGLQAKGIGTSYVDARDLVKTDDRFGHAKVNFKTTNKLIQDHFKSHD
DIKVITGFIGSTEKGETTTVGRSGSDYTASIFAAALGAEQVEIWTDVSGVMTADPRLVYT
AFTIPQLSYNEAMELSHFGAKVVFPATMQPAMKEDIPIYIKNTFKPDEAGTRISKDSGEG
KIIKGISSMDNISILNVQGPGLVEVVGVSQRFFGTLANNGINIILISQASSEHSICVAIA
SKDASRAKSVIEEEFRYEIQSGEMDEIQIVPDMAVIAVVGENMQHNPGASGRMFQALGRN
NVNVAAIAQGSSELNISAVITQADLQKALNALHEAFFLSDYKVLHLFLVGVGLIGKALTK
MIHQQLKNLQEENMLDIQIHGMANSRYMKFHEDGFDLATVGPPDENDEPMDMDKFIGTMT
EMNFSNSVFVDCTASQDVADIYEQILDSKVGIVTPNKKANSGPLETYKKLKKLAGQRGVR
FFYETNVAAGLPVINTLQDLMLSGDHVHRIEAVLSGSMNYIFSELEKGMPFSEVVAQAKE
KGYTEPDPRDDLSGMDVARKILILGREAGQDLHFEDVEIQSMVPEDCEDAASVPEFFKKL
QKHDGHFQQLLDEANAKGEKLRFMATLENGKAKVGLNSLDSEHPFFTLKGSDNMILFTTE
RYNDFPMIVRGPGAGADVTAAGVFADIIRLGNYSR