Protein Info for Echvi_1912 in Echinicola vietnamensis KMM 6221, DSM 17526

Updated annotation (from data): Nitrate reductase (EC 1.7.99.4)
Rationale: Specifically important for utilizing Sodium nitrate. Automated validation from mutant phenotype: the predicted function (1.7.99.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Uncharacterized anaerobic dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1173 PF04879: Molybdop_Fe4S4" amino acids 8 to 59 (52 residues), 63 bits, see alignment (E = 8.3e-21) PF00384: Molybdopterin" amino acids 64 to 492 (429 residues), 219.3 bits, see alignment E=3.1e-68 PF01568: Molydop_binding" amino acids 584 to 693 (110 residues), 118.2 bits, see alignment E=7.4e-38 PF07992: Pyr_redox_2" amino acids 708 to 997 (290 residues), 188.9 bits, see alignment E=5.8e-59 PF13738: Pyr_redox_3" amino acids 733 to 958 (226 residues), 35.2 bits, see alignment E=3.4e-12 PF00070: Pyr_redox" amino acids 850 to 929 (80 residues), 58.6 bits, see alignment 3e-19 PF18267: Rubredoxin_C" amino acids 1021 to 1093 (73 residues), 84 bits, see alignment 2.4e-27 PF04324: Fer2_BFD" amino acids 1116 to 1164 (49 residues), 42.7 bits, see alignment (E = 2.3e-14)

Best Hits

KEGG orthology group: None (inferred from 64% identity to fjo:Fjoh_4633)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FXZ3 at UniProt or InterPro

Protein Sequence (1173 amino acids)

>Echvi_1912 Nitrate reductase (EC 1.7.99.4) (Echinicola vietnamensis KMM 6221, DSM 17526)
MSTAKPESYKSTCSYCGVGCGIIVNKDNKGRTTVEGDPDHPVNRGMLCSKGMNLHYVVED
KSDRLQYPQMRWGKSHPLERVDWDTALSRSAAVFKSIIKKHGPDAVAFYVSGQCLTEEYY
LVNKLSKGFLKTNNIDTNSRLCMSSAVMGYIKTLGEDSVPISYEDIELADCFLVAGANPA
WCHPILWRRVEKHKEENPDTKIIVVDPRVTQSCSLADLHLQLNPGTDEVLYLAIGRCLIE
NGDIDVDFIQHHADGFDAYKELVMETSLESAAATCGVPVEDIKLAASYIGDAKGFLSLWA
MGLNQSAEGVNKNLALIDLNLITGHIGKPGSGPFSLTGQPNAMGGREVGGMATLLASHRN
LMNPEHRQEVADFWGVDSLPDQPGKTATQIFEGLEEGSIKALWIICTNPLVSMPDARRVE
AAMKKAKFVVVQDISAKAEAIPYADLVLPAAGWGEKEGTMTNSERRISYLNKFTDAPGEA
LPDAEILLKFAKKMNYSGFDFKNMAEVYAEYCQLTKGTNIDISGLDYDYLKSNGTVQWPF
TDKSKGGTARLFTDKKFYTPNGRAQILANGPKNKTELPSDDYPLILTTGRIRDQWHTMTR
TGKVSKLNKHIPTPFLEIHPEDAAERNLKDGETVLISGKRGEVRVHAQITDKIKKGVVFL
PMHWGKILQNDFSRTNNLTGNGIDPVSKQPDFKFSTVQVAKYVKPRQKIVIVGAGAAAYR
FINTYREFNQNDDIHVFSKEKHPFYNRVLLPDYVNRHKAWEDLLKFKNQGDMEKLNIQLY
VENGIESIDRSNKTVTDEKGRVHHYDTLILATGSRAFVPPDMPMHLPGTFTMRSRYDADK
LKEYLNYEGNVLIAGGGLLGLELAAALREINVGVTIVQLGSRLMERQLDPMASSLLRERI
EEMGVKLYMNNQIAHLDSHEATKEVDVRLKSGQEIHCNAVVLAIGTRPNMELAKSAGLTC
GRGIKVNDYLQTSDPSIFAMGEIAEHKGKLNGITAAAEMQADIAARFITGDLLSLYKGSI
PMNILKFADLDLCSIGIPEVPANGEGYEEILLIDTAQTYYKKCIVHHDRLVGAILMGDKS
EFAEFKGLIEEKTELSKKRQELLRGNANKEEMIGEMVCSCGNVGTGNISKAIQNGCHEFR
QLCQQTGAGLGCGSCKPEVKSILETELPALVKA