Protein Info for Echvi_1669 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Beta-galactosidase/beta-glucuronidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 828 signal peptide" amino acids 1 to 42 (42 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 52 to 201 (150 residues), 103.6 bits, see alignment E=3.6e-33 PF13364: BetaGal_ABD2" amino acids 104 to 181 (78 residues), 25.5 bits, see alignment E=5.5e-09 PF00703: Glyco_hydro_2" amino acids 206 to 316 (111 residues), 70.7 bits, see alignment E=5.4e-23 PF02836: Glyco_hydro_2_C" amino acids 324 to 477 (154 residues), 103.7 bits, see alignment E=3.3e-33 PF16355: DUF4982" amino acids 649 to 708 (60 residues), 79.5 bits, see alignment 4.2e-26 PF18565: Glyco_hydro2_C5" amino acids 722 to 822 (101 residues), 125.7 bits, see alignment E=1.9e-40

Best Hits

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FVL1 at UniProt or InterPro

Protein Sequence (828 amino acids)

>Echvi_1669 Beta-galactosidase/beta-glucuronidase (Echinicola vietnamensis KMM 6221, DSM 17526)
MSYFTYNFNGTVKGQIINTMQKFLSFILVFSLAVVSWNCASSEDKERQVIDFNYGWRFQL
GDHPNAISEDFDVSNWRELNLPHDWSIEGDFSEDHPTKPEGGALPAGIGWYRKAFKLPTE
AKEQSIWIEFDGVYRNGEVWINGHRLGKRPNGYSSFKYDLGEHLNYGDKVNVLAVRVDNS
EQPNSRWYTGSGIYRNVRLIRTGKVHVEHWGTYVTTPEITDSSAVVNLEVMVKNDGFNER
KLTVRSTILDADGEAVTEEEQPLVLGKGESTDVTQRFTVPSPKLWSTDEPYLYQVVTQVY
AGMQLMDDYVTPLGIRYFNFDAQKGFSLNGKRMKILGVCNHHDLGALGAAVNKRAIERRL
EILKEMGVNAIRTAHNPPAPELLQLCDEMGFIVQDEAFDVWKKKKVDADSHLFWDQWHRR
DLEDLILRDRNHPSIMMWSIGNEIREQFDSTGISITKELVRIVKELDTTRVVTCALTENI
PSKNFIYQSKALDLLGFNYKHKDHKNFPKWYPGEKLIATENMSALATRGHYDLPSDTIMR
WPQAHDKPLETGNEDLTVSAYDQVSAYWGSTHEETWKSIKDQDFMAGLFVWTGFDYLGEP
IPYPYPARSSYFGIVDLAGFPKDAYYMYQSEWTADTVLHVFPHWNWEAGQEVDVWAYYNQ
ADEVELFLNGESLGIKQKEGDDLHVMWRTPFEPGTLKAVARKDGKKVAEKKVTTAGDAQK
VTLSPDRKTIKADGKDLSFITVSICDMDGNIVPNADNMVNFEIQGEGKIMGVDNGYQASL
EPFKANYRKAFKGKCLLIVQSAREAGEISIRATSEHLQPGEIVLETVD