Protein Info for Echvi_1300 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: delta-1-pyrroline-5-carboxylate dehydrogenase, group 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 543 TIGR01236: 1-pyrroline-5-carboxylate dehydrogenase" amino acids 12 to 539 (528 residues), 804.1 bits, see alignment E=3e-246 PF00171: Aldedh" amino acids 56 to 512 (457 residues), 353.8 bits, see alignment E=6.1e-110

Best Hits

Swiss-Prot: 55% identical to PUT2_AGABI: Delta-1-pyrroline-5-carboxylate dehydrogenase (pruA) from Agaricus bisporus

KEGG orthology group: K00294, 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12] (inferred from 73% identity to mtt:Ftrac_3126)

MetaCyc: 49% identical to Delta1-pyrroline-5-carboxylate dehydrogenase, mitochondrial subunit (Homo sapiens)
RXN-14116 [EC: 1.2.1.88]; 1.2.1.- [EC: 1.2.1.88]

Predicted SEED Role

"Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization (EC 1.5.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FXV6 at UniProt or InterPro

Protein Sequence (543 amino acids)

>Echvi_1300 delta-1-pyrroline-5-carboxylate dehydrogenase, group 1 (Echinicola vietnamensis KMM 6221, DSM 17526)
MLKGFFNVPEPKNEPVFDYAPGTPARAKLQAALQEARSKEVDVPMYIGSEEVRTGNKIPL
SPPHDHQHLLGHFHEGDKSHVEQAINAALGAKEAWETMEWEQRAAIFLKAADLIAGPYRY
KMNAATMLGQSKNAFQAEIDSACEIVDFLRFNVKYMTEIYKQQPPISGDGVWNRLEQRPL
EGFVFALTPFNFTAIAGNLPTAPAMMGNTVVWKPAYTQIYTANLLMQVFREAGVPDGVIN
LVYVDGPAAGEVIFEHPEFAGIHFTGSTAVFQTIWKTIGNNIEKYKSYPRIVGETGGKDF
VIAHKSADAKQLATGLVRGAFEFQGQKCSAASRAYIPSNLWEDVKKYMQEDLASIKMGGP
EDFSNFINAVIDEKSFDKIAKYIDTAKSDGLEVVAGGHYDKSKGYFVEPTVLLTKDPMYT
TMCEEIFGPVLTIYVYQEDHFEEALELVDQTSPYGLTGAIFSHDRYAAQLATQKLRNAAG
NFYINDKPTGAVVGQQPFGGARKSGTNDKAGAMINMLRWVSPRTIKETFVTPTDYRYPFL
GED