Protein Info for Echvi_0979 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: siroheme synthase, N-terminal domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 190 TIGR01470: siroheme synthase, N-terminal domain" amino acids 5 to 174 (170 residues), 137.1 bits, see alignment E=3.2e-44 PF13241: NAD_binding_7" amino acids 7 to 118 (112 residues), 97.8 bits, see alignment E=5.4e-32 PF14824: Sirohm_synth_M" amino acids 124 to 148 (25 residues), 32.9 bits, see alignment (E = 3.6e-12)

Best Hits

KEGG orthology group: K02304, precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 4.99.1.4] (inferred from 67% identity to zpr:ZPR_3629)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.3.1.76, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.76 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FX74 at UniProt or InterPro

Protein Sequence (190 amino acids)

>Echvi_0979 siroheme synthase, N-terminal domain (Echinicola vietnamensis KMM 6221, DSM 17526)
MNELYPVFLKVAQLDTLIVGGGNVGLEKLSFLLKSSPKAKVTVLAKEISAEIRAIAAKND
RLQLIDAAYDKSYLENRHIVIGATDDKEVNKQIQQEAKAKHLLVNIADTPELCDFYLGGI
VTKGNVKIAISTNGKSPTTAKRLRQFLEEVIPDDINEMVENIHQYRDTLKGDFEYKVEAL
NKLTKELLVK