Protein Info for Echvi_0738 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Acyl-CoA dehydrogenases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 79 to 187 (109 residues), 104 bits, E=1.6e-33
• PF02770: Acyl-CoA_dh_M
  • amino acids 193 to 286 (94 residues), 79.2 bits, E=5.6e-26
• PF00441: Acyl-CoA_dh_1
  • amino acids 303 to 445 (143 residues), 118.3 bits, E=8.4e-38
• PF22924: ACOX_C_alpha1
  • amino acids 310 to 444 (135 residues), 29.3 bits, E=1.8e-10
• PF08028: Acyl-CoA_dh_2
  • amino acids 313 to 434 (122 residues), 39.4 bits, E=1.8e-13

Best Hits

Swiss-Prot: 48% identical to ACOX4_ARATH: Acyl-coenzyme A oxidase 4, peroxisomal (ACX4) from Arabidopsis thaliana

KEGG orthology group: K00252, glutaryl-CoA dehydrogenase [EC: 1.3.99.7] (inferred from 80% identity to dfe:Dfer_5685)

MetaCyc: 48% identical to ACX4 (Arabidopsis thaliana col)
Acyl-CoA oxidase. [EC: 1.3.3.6]; 1.3.3.6 [EC: 1.3.3.6]; 1.3.3.6 [EC: 1.3.3.6]; 1.3.3.6 [EC: 1.3.3.6]

Predicted SEED Role

"Glutaryl-CoA dehydrogenase (EC 1.3.99.7)" (EC 1.3.99.7)

MetaCyc Pathways

• fatty acid β-oxidation II (plant peroxisome) (4/5 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• (5Z)-dodecenoate biosynthesis II (4/6 steps found)
• methyl ketone biosynthesis (engineered) (4/6 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (4/7 steps found)
• fatty acid β-oxidation VII (yeast peroxisome) (2/5 steps found)
• 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
• fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) (1/5 steps found)
• propanoyl-CoA degradation II (1/5 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (2/10 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (3/13 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (3/14 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (1/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (1/12 steps found)
• jasmonic acid biosynthesis (4/19 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Fatty acid metabolism
• Lysine degradation
• Tryptophan metabolism
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.7

Use Curated BLAST to search for 1.3.3.6 or 1.3.99.7

Sequence Analysis Tools

See L0FT02 at UniProt or InterPro

Protein Sequence (452 amino acids)

>Echvi_0738 Acyl-CoA dehydrogenases (Echinicola vietnamensis KMM 6221, DSM 17526)
MKNLLQSFSSIKSLIKNIDFAKLNQLSQKVDLNEMVGIVSQMSESDLAKMMKMMKGGGKK
RELPPVNGDFYSLEKTLPPHEQEIVAKVRDFMEKEVRPIANEYWNKGHFPMHIIPKMAEL
GIAGLTYKGYGCPGHSALLEGFLAMEMARVDTSISTFFGVQSGLAMGSIYVCGSEEQKQE
WLPKMQKMDVIGAFGLTEPKVGSGVAGGLTTTCKREGDEWVINGQKKWIGNATFSDITVI
WARDLDDQQVKGFIVRKDNPGFHPEKIENKMALRTVQNALITMKDCRVPESDRLQNANSF
KDTSEILRLTRAGVAWQAVGCGRGAYEAALRYTNERKQFGRPIAGFQLVQDLLVTMLGDL
TAMQTMVYRLSKMQDAGELKDEHASLAKVFCTLRMRTIVDHSRELFGGNGILLEYDIARF
VADAEAIYSYEGTKEINSLIVGRAITGHSAFV