Protein Info for Echvi_0515 in Echinicola vietnamensis KMM 6221, DSM 17526

Updated annotation (from data): Ribulokinase (EC 2.7.1.16)
Rationale: Specifically important for utilizing L-Arabinose. Automated validation from mutant phenotype: the predicted function (RXN0-5116) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: L-ribulokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01234: ribulokinase
  • amino acids 4 to 552 (549 residues), 614.6 bits, E=8.4e-189
• PF00370: FGGY_N
  • amino acids 6 to 190 (185 residues), 36.3 bits, E=7.2e-13
• PF02782: FGGY_C
  • amino acids 290 to 506 (217 residues), 131.4 bits, E=5.9e-42

Best Hits

KEGG orthology group: K00853, L-ribulokinase [EC: 2.7.1.16] (inferred from 64% identity to hhy:Halhy_0353)

Predicted SEED Role

"Ribulokinase (EC 2.7.1.16)" in subsystem L-Arabinose utilization (EC 2.7.1.16)

MetaCyc Pathways

• L-arabinose degradation I (4/4 steps found)
• D-arabinose degradation I (1/2 steps found)
• ribitol degradation I (1/2 steps found)
• D-arabinose degradation V (1/3 steps found)
• superpathway of pentose and pentitol degradation (11/42 steps found)

KEGG Metabolic Maps

• Pentose and glucuronate interconversions

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.16

Use Curated BLAST to search for 2.7.1.16

Sequence Analysis Tools

See L0FS84 at UniProt or InterPro

Protein Sequence (562 amino acids)

>Echvi_0515 Ribulokinase (EC 2.7.1.16) (Echinicola vietnamensis KMM 6221, DSM 17526)
MNDKFVIGLDYGSDSVRAVIVNTGNGEVKGSHVFWYPRWKAGKYCDPVNNQFRQHPLDHL
EGLEETIREVIKESGVPADQIKGICVDTTGSSPMAVDQQGKPLALSPEFAENPNAMMVLW
KDHTAIKEADEINELARTWGGEDYTKYEGGIYSSEWFWAKILHVIREDDAVAQAAYSWME
HCDVITAELIGADNPLDVKRSRCAAGHKALWHESWDGLPPKEFLSKLDPRLADLRDRLYT
ETFTSDLPAGNLSAEWAKKLGLSTDTVIAVGTFDAHAGAVGGEVTENTLVKVMGTSTCDI
MVATHEAIGDNLVKGICGQVDGSVIPGTVGLEAGQSGFGDVLAWFKHLVVKPTAALIRAS
NVLDEEKKEALIHEIDSQLLIKLSEEAIQIPLSETAPVALDWVNGRRTPDANQALKGAVM
GLNMGTDAARVFKALVESICFGSKKIVDRFREEGVAIDTVIGMGGVAKKSKLVMQTMADV
LNMPIKIATSDQAPALGAAMYASVAAGIHPTTEAAIAAMTNGFDKVYEPIPENVEVYKAL
YAKYAEFGAFVEGQTSPVLADQ