Protein Info for Echvi_0118 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Lysyl-tRNA synthetase (class II)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 572 TIGR00499: lysine--tRNA ligase" amino acids 6 to 502 (497 residues), 624.5 bits, see alignment E=6.3e-192 PF01336: tRNA_anti-codon" amino acids 54 to 137 (84 residues), 50.5 bits, see alignment E=2.4e-17 PF00152: tRNA-synt_2" amino acids 162 to 498 (337 residues), 311.7 bits, see alignment E=7.3e-97

Best Hits

Swiss-Prot: 62% identical to SYK_BACFR: Lysine--tRNA ligase (lysS) from Bacteroides fragilis (strain YCH46)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 74% identity to phe:Phep_1559)

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0FUR0 at UniProt or InterPro

Protein Sequence (572 amino acids)

>Echvi_0118 Lysyl-tRNA synthetase (class II) (Echinicola vietnamensis KMM 6221, DSM 17526)
MQLLSEQEIERRKDREELMKLGINPYPAIQFPINASAEDIHKNYENNKNDYKDISIAGRL
MSRRIMGSASFAEIQDSSGRLQVYVRRDDICPGEDKTLYNKVFKKLLGIGDFIGVKGYIF
TTQTGEISLHVTELTVLSKSVKPLPVVKRDEEGNVYDGFTDPELRYRQRYVDLTVNPEKK
NVFLTRSRIITNMRKYFDDHGWLEVETPILQAVHGGAAARPFETHHNSLDMPLYLRIANE
LYLKRLIVGGFDGVYEFGKMFRNEGMDRTHNPEFTSMEIYVAYKDYVWMMEMVEDLLEKV
TLSVHETSKVKVGEREIDFAGPYRRLTMFDSIKEYAGVDVSKMDETALRKVCADFGIEVD
DSMGKGKLIDEIFGEKVEEHLIQPTYITDYPIEMTPLAKKHRTEEGLVERFELFVNGKEI
ANAYTELNDPIDQRERFEDQLKLAERGDDEAMAMDEDFLRALEYGMPPTSGLGIGIDRLT
MMLTDNSTIQEVLFFPQMRPEKKVKIATDEDFIALGIDAGLIPAIRELNIHTIEQLKEQD
VNKLFNDVCGKRKKLKLEVKNPSKEDVANWLA