Protein Info for EX28DRAFT_4357 in Enterobacter asburiae PDN3

Annotation: Alpha-glucosidases, family 31 of glycosyl hydrolases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 674 PF13802: Gal_mutarotas_2" amino acids 60 to 158 (99 residues), 33.6 bits, see alignment E=7.4e-12 PF01055: Glyco_hydro_31_2nd" amino acids 262 to 554 (293 residues), 156.5 bits, see alignment E=1.8e-49 PF21365: Glyco_hydro_31_3rd" amino acids 579 to 661 (83 residues), 79.6 bits, see alignment E=3e-26

Best Hits

Swiss-Prot: 84% identical to SQASE_ECOLI: Sulfoquinovosidase (yihQ) from Escherichia coli (strain K12)

KEGG orthology group: K01811, putative family 31 glucosidase (inferred from 85% identity to ctu:CTU_41780)

MetaCyc: 84% identical to sulfoquinovosidase (Escherichia coli K-12 substr. MG1655)
RXN-17700 [EC: 3.2.1.199]; 3.2.1.199 [EC: 3.2.1.199]

Predicted SEED Role

"Alpha-glucosyltransferase YihQ"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.199

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (674 amino acids)

>EX28DRAFT_4357 Alpha-glucosidases, family 31 of glycosyl hydrolases (Enterobacter asburiae PDN3)
MRTLHNINLKNNENGFTLSWENRLILSHTADAPCLWIGAGEADIEMFRGNFSIKDKLDEK
IALTDATVTQQSAGWAIRFTRGDAVSATLLVGVDADGRLELKLKNDAPGHNRIWLRLAAE
PEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQTYVTWQADCKENAGGDYYWTFFPQ
PTFVSTQKYYCHVDNSCYMNFDFSAPDFHELAFWEDNATLRFECAKTYVDLLEKLTGLLG
RQPELPDWVYDGVTLGIQGGTEVCQQKLDAMRQGGVKVNGIWAQDWSGIRMTSFGKRVMW
NWKWNSELYPQLDERIARWKQEGVQLLSYINPYVASDKDLCEEAATRGYLTKDADGKDYH
VEFGEFYAGVIDLTNPEAYGWYKEVIKKNLIALGCGGWMADFGEYLPTDTFLHNGVSAEI
MHNAWPALWAKCNYEALEETGKLGEILFFMRAGYTGSQKHSMMMWAGDQNVDWSLDDGLA
SVVPAALSLAMTGHGLHHSDIGGYTTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGD
NWQFDGDAETIAHFARMTTVFTTLKPYIKAAVAQNAKSGLPVMRPLFLHYEDDARAYTLK
YQYLFGRDLLVAPVHEEGRRDWTLYLPQDSWVNAWTGEICQGGDVTVDAPLGKPPVFYRQ
HSDWADLFSTLRHI