Protein Info for EX28DRAFT_4294 in Enterobacter asburiae PDN3

Annotation: 4-aminobutyrate aminotransferase, prokaryotic type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00700: 4-aminobutyrate transaminase
  • amino acids 9 to 420 (412 residues), 640.1 bits, E=6.5e-197
• PF00202: Aminotran_3
  • amino acids 24 to 417 (394 residues), 431.2 bits, E=1.7e-133

Best Hits

Swiss-Prot: 70% identical to PUUE_ECOLI: 4-aminobutyrate aminotransferase PuuE (puuE) from Escherichia coli (strain K12)

KEGG orthology group: K00823, 4-aminobutyrate aminotransferase [EC: 2.6.1.19] (inferred from 97% identity to enc:ECL_04821)

MetaCyc: 72% identical to 4-aminobutyrate aminotransferase monomer (Cupriavidus necator H16)
4-aminobutyrate transaminase. [EC: 2.6.1.19]; 2.6.1.- [EC: 2.6.1.19]

Predicted SEED Role

"Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)" (EC 2.6.1.19)

MetaCyc Pathways

• superpathway of L-arginine and L-ornithine degradation (12/13 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (10/11 steps found)
• 4-aminobutanoate degradation I (2/2 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• superpathway of 4-aminobutanoate degradation (2/3 steps found)
• 4-aminobutanoate degradation II (1/2 steps found)
• homotaurine degradation (1/2 steps found)
• GABA shunt I (2/4 steps found)
• GABA shunt II (2/4 steps found)
• 4-aminobutanoate degradation V (2/7 steps found)
• nicotine degradation I (pyridine pathway) (5/17 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Butanoate metabolism
• Glutamate metabolism
• Propanoate metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.19

Use Curated BLAST to search for 2.6.1.19

Protein Sequence (421 amino acids)

>EX28DRAFT_4294 4-aminobutyrate aminotransferase, prokaryotic type (Enterobacter asburiae PDN3)
MKNNELNDRRLQATPRGIGVMCNFYADKAENATVWDVEGNEYIDFAAGIAVLNTGHRHPK
VISAIEKQLHAFTHTAYQIVPYEGYVALAERINQRVPIDGPAKTAFFSTGAEAVENAVKI
ARAYTKRPGLITFGGAFHGRTFMTMALTGKVAPYKLGFGPFPGSVYHAQYPNELHGVSTA
EALKSLERIFKADIAPDQVAAIILEPVQGEGGFNIAPADFMQALRALCDTHGILLIADEV
QSGFARTGKLFSMEHHCVKPDLITMAKSLAGGMPLSAVSGRAEVMDVPAPGGLGGTYAGN
PLAIAAAHAVLDVIDEEDLCTRSAHLGHHLVEVLNKAKEHCPYIADIRAQGSMVAVEFND
PRTGQPSPEFTRQVQDRALQEGLLLLSCGVYGNVIRFLYPLTIPEAQFRKALDIISASLT
R