Protein Info for EX28DRAFT_4271 in Enterobacter asburiae PDN3

Annotation: glycerol-3-phosphate acyltransferase (EC 2.3.1.15)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03703: glycerol-3-phosphate O-acyltransferase
  • amino acids 11 to 799 (789 residues), 1172.9 bits, E=0
• PF01553: Acyltransferase
  • amino acids 282 to 424 (143 residues), 104 bits, E=5.8e-34
• PF19277: GPAT_C
  • amino acids 435 to 771 (337 residues), 374.5 bits, E=5.8e-116

Best Hits

Swiss-Prot: 97% identical to PLSB_ENT38: Glycerol-3-phosphate acyltransferase (plsB) from Enterobacter sp. (strain 638)

KEGG orthology group: K00631, glycerol-3-phosphate O-acyltransferase [EC: 2.3.1.15] (inferred from 98% identity to enc:ECL_00298)

MetaCyc: 93% identical to glycerol-3-phosphate 1-O-acyltransferase (Escherichia coli K-12 substr. MG1655)
Glycerol-3-phosphate O-acyltransferase. [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]

Predicted SEED Role

"Glycerol-3-phosphate acyltransferase (EC 2.3.1.15)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.3.1.15)

MetaCyc Pathways

• superpathway of phospholipid biosynthesis III (E. coli) (11/12 steps found)
• phosphatidylglycerol biosynthesis I (6/6 steps found)
• phosphatidylglycerol biosynthesis II (6/6 steps found)
• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• superpathway of cardiolipin biosynthesis (bacteria) (10/13 steps found)
• oleate biosynthesis III (cyanobacteria) (2/3 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
• palmitoyl ethanolamide biosynthesis (2/6 steps found)
• superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
• stigma estolide biosynthesis (2/7 steps found)
• anandamide biosynthesis II (2/8 steps found)
• anandamide biosynthesis I (4/12 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15

Protein Sequence (806 amino acids)

>EX28DRAFT_4271 glycerol-3-phosphate acyltransferase (EC 2.3.1.15) (Enterobacter asburiae PDN3)
MSGWPRIYYKLLNLPLSILVKSKSIPAEPALELGLDTSRPIMYVLPYNSKADLLTLRAQC
LAHDLPDPLEPLEVDGTLLPRYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPDLDV
QMVPVSVMFGRRPGREKGEENPPLRMLNGIQKFFAVSWLGRDSFVRFSPSVSLRRMADEH
GTDKIIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKLLASKAIARAVEDEARSKKIS
HEKAQQNAIALMEEIAANFSYEMIRLSDRILGFTWNRLYQGINVHNAERVRQLAHDGHEI
VYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNK
LYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLV
PIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLN
HHVPEWRESIDPIEAIRPAWLTPTVNGIASELMVRINNAGAANAMNLCCTALLASRQRSL
TREQLTEQLDCYLDMMRNVPYSVDSTVPSATASELIDHALQMNKFEVEKDTIGDIIILPR
EQAVLMTYYRNNITHMLMLPSLMAAIITQHRSISRQELLRHVETLYPMLKAELFLRWSKD
ELAGELDKLTEELRRQGLIAVSNDELHINPSRSRTLQLLAAGARETLQRYAITFWLLSAN
PSINRGTLEKESRTLAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDTGDAEPEET
LKVYQMLAELITSDVRLTIESATQGE