Protein Info for EX28DRAFT_3704 in Enterobacter asburiae PDN3

Annotation: Membrane protein required for beta-lactamase induction

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 signal peptide" amino acids 1 to 15 (15 residues), see Phobius details transmembrane" amino acids 39 to 58 (20 residues), see Phobius details amino acids 64 to 87 (24 residues), see Phobius details amino acids 144 to 168 (25 residues), see Phobius details amino acids 191 to 211 (21 residues), see Phobius details amino acids 266 to 283 (18 residues), see Phobius details PF17113: AmpE" amino acids 1 to 284 (284 residues), 520.4 bits, see alignment E=6.5e-161

Best Hits

Swiss-Prot: 81% identical to AMPE_ECOLI: Protein AmpE (ampE) from Escherichia coli (strain K12)

KEGG orthology group: K03807, AmpE protein (inferred from 97% identity to enc:ECL_00907)

Predicted SEED Role

"AmpE protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (284 amino acids)

>EX28DRAFT_3704 Membrane protein required for beta-lactamase induction (Enterobacter asburiae PDN3)
MTLFTMLLVIIAERLFKLGEHWHLDHRLEVLFRRIKHFSMLRTLLMMAGVMVITFLLLRS
LYGLFFNVPLLVVWILLGVLCIGAGKVRLHYHAYLKAASRDDAHARGAMASELTMIHGVP
PDCDEREFLRELQNALLWINFRYYLAPLFWFVVGGPLGPVLLMGYAFLRAWQTWLARYLT
PHERLQSGIDAILHVLDWLPVRLVGVVYALIGHGEKALPAWFASLADRHTSQYQVLTRLA
QFSLAREPHTDKIETPKAAVSMAKKTSFVVVVIVALLTIYGTLV