Protein Info for EX28DRAFT_3598 in Enterobacter asburiae PDN3

Annotation: nicotinamide-nucleotide adenylyltransferase, NadR type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 410 PF01381: HTH_3" amino acids 7 to 59 (53 residues), 34.4 bits, see alignment 2.8e-12 TIGR01526: nicotinamide-nucleotide adenylyltransferase" amino acids 64 to 395 (332 residues), 531.4 bits, see alignment E=8.4e-164 TIGR00125: cytidyltransferase-like domain" amino acids 66 to 136 (71 residues), 67.3 bits, see alignment E=1e-22 PF01467: CTP_transf_like" amino acids 69 to 107 (39 residues), 21.7 bits, see alignment 3e-08 PF13521: AAA_28" amino acids 234 to 394 (161 residues), 110.9 bits, see alignment E=1.2e-35

Best Hits

Swiss-Prot: 94% identical to NADR_SALTY: Trifunctional NAD biosynthesis/regulator protein NadR (nadR) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K06211, HipB family transcriptional regulator, involved in the regulation of NAD biosynthesis (inferred from 99% identity to enc:ECL_00801)

MetaCyc: 93% identical to DNA-binding transcriptional repressor/NMN adenylyltransferase NadR (Escherichia coli K-12 substr. MG1655)
Nicotinamide-nucleotide adenylyltransferase. [EC: 2.7.7.1]; Ribosylnicotinamide kinase. [EC: 2.7.7.1, 2.7.1.22]

Predicted SEED Role

"NadR transcriptional regulator / Nicotinamide-nucleotide adenylyltransferase, NadR family (EC 2.7.7.1) / Ribosylnicotinamide kinase (EC 2.7.1.22)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation or PnuC-like transporters (EC 2.7.1.22, EC 2.7.7.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.22 or 2.7.7.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (410 amino acids)

>EX28DRAFT_3598 nicotinamide-nucleotide adenylyltransferase, NadR type (Enterobacter asburiae PDN3)
MSSFDYIKTAIRQKGCTLQQVADASGMTKGYLSQLLNAKIKSPSAQKLEALHRFLGLEFP
RMQKNIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGYDETRDRQLFEDSAMSQQPTV
PDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNGIKAFMEEKGIAPNWIYTSEE
SDAPQFREHLGIETVLIDPKRTFMNISGAQIRENPFRYWDYIPTEVKPFFVRTVAILGGE
SSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDEMALQYSDYDKIALGHAQYIDFAVK
YANKVAFIDTDFVTTQAFCKKYEGREHPFVQALIDEYRFDLVILLENNTPWVADGMRSLG
SSVDRREFQTMLVEMLNENNVEFVHVEESDYDSRFLRCVELVKEMMGEQG