Protein Info for EX28DRAFT_3209 in Enterobacter asburiae PDN3

Annotation: luciferase family oxidoreductase, group 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 transmembrane" amino acids 53 to 67 (15 residues), see Phobius details TIGR03558: luciferase family oxidoreductase, group 1" amino acids 8 to 328 (321 residues), 448.6 bits, see alignment E=6.5e-139 PF00296: Bac_luciferase" amino acids 8 to 305 (298 residues), 134 bits, see alignment E=4e-43

Best Hits

Swiss-Prot: 93% identical to YHBW_ECOL6: Luciferase-like monooxygenase (yhbW) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 99% identity to enc:ECL_04542)

Predicted SEED Role

"Luciferase-like monooxygenase (EC 1.14.-.-)" (EC 1.14.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.-.-

Use Curated BLAST to search for 1.14.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (335 amino acids)

>EX28DRAFT_3209 luciferase family oxidoreductase, group 1 (Enterobacter asburiae PDN3)
MTDKTIPFSVLDLAPIPQGSSAREAFSHSLDLAQLAEKRGYHRYWLAEHHNMVGIASAAT
SVLIGYLAANTTTLHLGSGGVMLPNHAPLVIAEQFGTLNTLYPGRIDLGLGRAPGSDQPT
MRALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPGYGEKIPVWLLGSSLYSAQLA
AQLGLPFAFASHFAPDMLHQALHLYRTNFKPSERLEKPYAMVCINIIAADSNRDAEFLFT
SMQQAFVKLRRGETGQLPPPVENMHQLWSASEQYGVQQALSMSLVGDKAKVRHGLDAVLR
ETQADEIMVNGQIFDHQARLHSFELAMQVKEELVG