Protein Info for EX28DRAFT_2607 in Enterobacter asburiae PDN3

Annotation: Homocysteine/selenocysteine methylase (S-methylmethionine-dependent)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 85% identical to MMUM_ECOLI: Homocysteine S-methyltransferase (mmuM) from Escherichia coli (strain K12)

KEGG orthology group: K00547, homocysteine S-methyltransferase [EC: 2.1.1.10] (inferred from 94% identity to enc:ECL_01102)

MetaCyc: 85% identical to CP4-6 prophage; homocysteine S-methyltransferase (Escherichia coli K-12 substr. MG1655)
Homocysteine S-methyltransferase. [EC: 2.1.1.10]

Predicted SEED Role

"Homocysteine S-methyltransferase (EC 2.1.1.10)" in subsystem Methionine Biosynthesis (EC 2.1.1.10)

MetaCyc Pathways

L-methionine salvage from L-homocysteine (2/3 steps found)
S-methyl-L-methionine cycle (1/2 steps found)
superpathway of L-methionine salvage and degradation (7/16 steps found)

KEGG Metabolic Maps

Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (310 amino acids)

>EX28DRAFT_2607 Homocysteine/selenocysteine methylase (S-methylmethionine-dependent) (Enterobacter asburiae PDN3)
MSQNNPLTAILEKQPFVVLDGAMATELEARGCNLADSLWSAKVLVENPELIREVHLDYYR
AGAQVAITASYQATPAGFAARGLDEAQSRALIGKSVELARKAREAYLAENPHAGTLLVAG
SVGPYGAYLADGSEYRGDYVRSAEEFTTFHRPRVEALLDAGADLLACETLPSFVEIKALA
ALLAEYPRARAWFSFTLRDSEHLSDGTPLREVVSALANSPQIVALGINCIALENTTAALK
HLQSLTSLPLVVYPNSGEHYDAVTKTWHHHGEACETLAGYLPQWLEVGAKLIGGCCRTTP
KDIAELNAKR