Protein Info for EX28DRAFT_2385 in Enterobacter asburiae PDN3
Annotation: choline/carnitine/betaine transport
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to BETT_ECOLI: High-affinity choline transport protein (betT) from Escherichia coli (strain K12)
KEGG orthology group: K02168, high-affinity choline transport protein (inferred from 96% identity to eco:b0314)MetaCyc: 96% identical to choline:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-99
Predicted SEED Role
"High-affinity choline uptake protein BetT" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (677 amino acids)
>EX28DRAFT_2385 choline/carnitine/betaine transport (Enterobacter asburiae PDN3) MTDLSQDREKDKINPVVFYTSAGLILLFSLTTIFFRDFSAEWIGRTLNWVSKTFGWYYLL AATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFAAGIGIDLMFFSVAEPVTQ YMQPPEGAGQTMEAARQAMVWTLFHYGLTGWSMYALMGMALGYFSYRYNLPLTIRSALYP IFGKKINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLNYGLSVLFDIPDSMAAKAALIAL SVIIATISVTSGVDKGIRVLSELNVALALGLILFVLFMGDTSFLLNALVLNVGDYVNRFM GMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFVGLFLARISRGRTIRQFVMGTLIIPFT FTLLWLSIFGNSALHEIIHGNATFAQEAMAHPERGFYSLLAQYPAFTFSASVATITGLLF YVTSADSGALVLGNFTSKLKDINSDAPNWLRIFWSVAIGLLTLGMLMTNGISALQNTTVI MGLPFSFVIFFVMAGLYKSLKVEDYRRVSASRDTAPRPMGAQDRLSWKKRLSRLMNYPGT RYTKQMMEAVCFPAMEEVAQELKLRGAYVELKNLPPEEGETLGHLDLLVHMGDEQNFVYQ IWPQQYSVPGFTYRARSGKSTYYRLETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNF IHLHREAPGNSVMFPDV