Protein Info for EX28DRAFT_2278 in Enterobacter asburiae PDN3

Annotation: Deoxyribodipyrimidine photolyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 470 PF00875: DNA_photolyase" amino acids 5 to 168 (164 residues), 139.5 bits, see alignment E=1.1e-44 PF03441: FAD_binding_7" amino acids 271 to 465 (195 residues), 292.3 bits, see alignment E=1.7e-91

Best Hits

Swiss-Prot: 74% identical to PHR_ECOLI: Deoxyribodipyrimidine photo-lyase (phrB) from Escherichia coli (strain K12)

KEGG orthology group: K01669, deoxyribodipyrimidine photo-lyase [EC: 4.1.99.3] (inferred from 85% identity to enc:ECL_03018)

MetaCyc: 74% identical to deoxyribodipyrimidine photolyase (Escherichia coli K-12 substr. MG1655)
Deoxyribodipyrimidine photo-lyase. [EC: 4.1.99.3]; 4.1.99.3 [EC: 4.1.99.3]; 4.1.99.3 [EC: 4.1.99.3]

Predicted SEED Role

"Deoxyribodipyrimidine photolyase (EC 4.1.99.3)" (EC 4.1.99.3)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (470 amino acids)

>EX28DRAFT_2278 Deoxyribodipyrimidine photolyase (Enterobacter asburiae PDN3)
MPTHLVWFRADLRVHDNIALAAACRDKDANVLALFMATPEQWRQHDMAPRQAALLRAYLT
DLQHSLAGKGIPLLYKEVGDFAAQLQTVQEICKQHKVTHLFYNYQYEFNEQQRDRQLEKM
LEGVVCEGFDDSVMLAPGSVMTGNHEMYKVFTPFKNAFIKRLNEALPECVAAPAVRGEAI
KELPELTFDYPQQPFDETLFPADEKAAIAQLRQFCKQDAASYDARRDFPAIEGTSRLSAC
LALGAISPRQCLHRLLAEQPQALEGGAGSVWLNELIWREFYRHLMTYHPDLCKHRPFIGW
TDNVRWQSNDRLLKAWQTGQTGYPIVDAAMRQLNETGWMHNRLRMITASFLVKDLLIDWR
IGERYFVSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGQKFDADGEFIRRWVP
ELNAIPAKAIHEPWAWADKQRVKLDYPRPIVDHKQARVATLAAYEAARKA