Protein Info for EX28DRAFT_2224 in Enterobacter asburiae PDN3

Annotation: Pectin methylesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01095: Pectinesterase
  • amino acids 195 to 339 (145 residues), 42.8 bits, E=1.4e-15

Best Hits

Swiss-Prot: 80% identical to YBHC_ECOLI: Putative acyl-CoA thioester hydrolase YbhC (ybhC) from Escherichia coli (strain K12)

KEGG orthology group: K01051, pectinesterase [EC: 3.1.1.11] (inferred from 96% identity to enc:ECL_02969)

Predicted SEED Role

"Pectinesterase (EC 3.1.1.11)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 3.1.1.11)

MetaCyc Pathways

• pectin degradation II (3/5 steps found)
• pectin degradation I (1/3 steps found)

KEGG Metabolic Maps

• Pentose and glucuronate interconversions
• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.11

Protein Sequence (427 amino acids)

>EX28DRAFT_2224 Pectin methylesterase (Enterobacter asburiae PDN3)
MNISRISRLALALAFGVTLSACSSTPPDQRPSEQVAPGTTSRPILSADEAKNFDRAHYFS
AMDPNAAPWTPSSINLPKQPDFVVGPAGAQGVTHTSIQAAVDAAITKHSASRQYIAILPG
EYEGTVYVPAAPGSITLYGLGEKAIDVKIGLAIDSEIDSNTWRHLVNPAGKYMPGKPAWY
MFDNCQSKRSATIGVMCSAVFWSQNNGLQLQNLTIQNTLGDSVDAGNHQAVALRSDGDQV
QINNVNILGRQNTFFVTNSGVQNTLQNNRLTRTLVTNSYVEGDVDLVSGRGAVVFDNTDF
RVVNSRTQQEGYVFAPATQSNLFYGFLAVNSRFNAAGDGVAQLGRSLDVDSATNGQVVIR
DSVINEGFNMAKPWADAAISKRPFSGNTGAVDDKGNVQRNLNDANFNRMWEYNNRGLGSK
VVAEPKQ