Protein Info for EX28DRAFT_2183 in Enterobacter asburiae PDN3

Annotation: Predicted SAM-dependent methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 PF05971: Methyltransf_10" amino acids 3 to 297 (295 residues), 465.6 bits, see alignment E=3.8e-144

Best Hits

Swiss-Prot: 86% identical to RLMF_ENT38: Ribosomal RNA large subunit methyltransferase F (rlmF) from Enterobacter sp. (strain 638)

KEGG orthology group: K06970, ribosomal RNA large subunit methyltransferase F [EC: 2.1.1.181] (inferred from 92% identity to enc:ECL_02924)

MetaCyc: 77% identical to 23S rRNA m6A1618 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11596 [EC: 2.1.1.181]

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51)" (EC 2.1.1.51)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.51

Use Curated BLAST to search for 2.1.1.181 or 2.1.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (303 amino acids)

>EX28DRAFT_2183 Predicted SAM-dependent methyltransferase (Enterobacter asburiae PDN3)
MTAQKPGLHPRNRHRSRYDMNALCLSCPPLQDYLVQTPAGEPSVNFADPQAVKMLNKALL
AHFYGVAHWDIPEGFLCPPVPGRADYVHHLADLLAEGNEGVVPQQATILDIGTGANLIYP
LIGAHEYQWRFTGSEIGDEAFASAQAIISANPGLSRAIRLRRQKDAASIFNGIIHKNESY
DATMCNPPFHDSAASARAGSERKRRNLGQAEDAALNFGGQQQELWCEGGEVAFILRMIAE
SKQFGRQVKWFTTLVSRGDNLPPLYRALTEAGAVKVVKKEMAQGQKQSRFIAWTFMDNHK
RRK