Protein Info for EX28DRAFT_1595 in Enterobacter asburiae PDN3

Annotation: phage replication protein O

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 PF04492: Phage_rep_O" amino acids 50 to 148 (99 residues), 85 bits, see alignment E=2.1e-28 TIGR01610: phage replication protein O, N-terminal domain" amino acids 52 to 148 (97 residues), 128.5 bits, see alignment E=5.4e-42

Best Hits

Swiss-Prot: 72% identical to VG15_BPPH8: Replication protein 15 (15) from Enterobacteria phage phi80

KEGG orthology group: None (inferred from 72% identity to ecd:ECDH10B_1331)

Predicted SEED Role

"Primosomal protein I" in subsystem DNA-replication

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (335 amino acids)

>EX28DRAFT_1595 phage replication protein O (Enterobacter asburiae PDN3)
MNTLEKAKAGLREQNRLSGAKKRSVIAELSMSNTAEIINFPHRTEQLGGRMADLSNGYTK
VANEIQQLKPRLRMSGREWQCFEAVIWLTYGWNKKQDRVTNTVIAELTGLSDSHVSDALK
SLADRKIIFSQKQGVMKTVGINTDLSAWILDKPKTGKVFPKSGKVLPKTGKTFPETVDTQ
DYNKNNNKISSSRNSDESRNQKTQKFLSRHPEAAAGIYTPAGKSWGSADDLKAARWIYDR
LLTVNASLSEPNWVEWANTIRLMRVQDKRTHYEICDLFQWANRDEFWKDNILSPSSLRKQ
WDQLTTKRLRATGAAKPSRGGIDLHNTDWIDGVLE