Protein Info for EX28DRAFT_1499 in Enterobacter asburiae PDN3

Annotation: Predicted glutamine amidotransferases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07722: Peptidase_C26
  • amino acids 8 to 224 (217 residues), 230 bits, E=2.9e-72
• PF00117: GATase
  • amino acids 95 to 241 (147 residues), 47.1 bits, E=2.3e-16

Best Hits

Swiss-Prot: 85% identical to PUUD_ECOLI: Gamma-glutamyl-gamma-aminobutyrate hydrolase PuuD (puuD) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 90% identity to eae:EAE_15930)

MetaCyc: 85% identical to gamma-glutamyl-gamma-aminobutyrate hydrolase (Escherichia coli K-12 substr. MG1655)
Gamma-glutamyl-gamma-aminobutyrate hydrolase. [EC: 3.5.1.94]

Predicted SEED Role

"Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94)" (EC 3.5.1.94)

MetaCyc Pathways

• superpathway of L-arginine and L-ornithine degradation (12/13 steps found)
• superpathway of ornithine degradation (8/8 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (10/11 steps found)
• putrescine degradation II (4/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.94

Protein Sequence (254 amino acids)

>EX28DRAFT_1499 Predicted glutamine amidotransferases (Enterobacter asburiae PDN3)
MENIMNKPVIGVVMCRNRLKGHETQTLQEKYLNAVINAGGLPIALPHALAEPELMAALLP
TLDGIYLPGSPSNVQPHLYGENGDEPNADPGRDLLSMALIAAALERRIPIFAICRGLQEL
VVATGGTLYRRLFEQNDLLEHREDAELPVELQYAPSHEVQVQEGGLLSQLIPGCNKFWVN
SLHGQGARTLGPRIRVEARSTDGLVEAVSVHDHPFALGVQWHPEWNSSEYALSRMLFEGF
ITACQSHVAEKQRL