Protein Info for EX28DRAFT_1441 in Enterobacter asburiae PDN3

Annotation: PAS domain S-box/diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 860 transmembrane" amino acids 24 to 45 (22 residues), see Phobius details amino acids 163 to 182 (20 residues), see Phobius details amino acids 262 to 285 (24 residues), see Phobius details PF05228: CHASE4" amino acids 64 to 216 (153 residues), 60 bits, see alignment E=4.4e-20 TIGR00229: PAS domain S-box protein" amino acids 312 to 380 (69 residues), 27.7 bits, see alignment E=2.4e-10 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 434 to 597 (164 residues), 139.5 bits, see alignment E=8.4e-45 PF00990: GGDEF" amino acids 438 to 594 (157 residues), 168.8 bits, see alignment E=1.3e-53 PF00563: EAL" amino acids 615 to 845 (231 residues), 241 bits, see alignment E=1.7e-75

Best Hits

KEGG orthology group: None (inferred from 84% identity to enc:ECL_02167)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (860 amino acids)

>EX28DRAFT_1441 PAS domain S-box/diguanylate cyclase (GGDEF) domain (Enterobacter asburiae PDN3)
MTLPLNNPGFTDTEPSKGLINKTFTYIVTLLISIFIAAIISLLIIKDNVNEISDTHDEFL
LRKALDTRQESMRSHLKDNAEWGDAYKYLHLNTDVHWAWDKQNLGKSLYDNFGYEGVFVL
SPEGSTRYSVVDGKLKLQDLERWLDKSTKDRLLRETSIKNGSPVSVLTLIDSVPAIVSAQ
WITTGDDSSVQTLPGKPSTMVFIDKLTARKLLTIGQDAGIQNVRTSPATVTSQINNQTEF
TLSTQNGDVKIIWDGENPGQALIIYFLPLLILSVVLTVSMTVILMRHIMHKARMLDENTF
LLEQARLNLITSEKRFRDVSETTSDWFWETDLSLRITWLSGRFSTITGYENSKWIGRRLD
ELFPSTSELSHDCARQRDLTERFEFKNCPYTHAQQTTSYCTLLAKFSAQPDGTVVLRGAA
TDVSQEVEATKRVEFLSRHDELTGLPNRYHIKEFLAGKLAKEDLNHYPFAMICLDLDKFK
PVNDIFGHSTGDALLGEVASRLKNCVRRGDFVARQGGDEFMLLLGNTSQQDQIEEVCRRI
VQELNRPFAIEGNDVAIGVSMGIALAPRDSSSANDLLRYADIALYQAKQSGRNRWVYYRP
DMSEKLTERRKLELELKTAIREEQLYLVYQPRYNLRYSTIDAVEALVRWQHPARGTIMPD
QFIPLAEETGLIISLSNWVIRKACAETKDKLPGLSVSVNISAIEFQASDLAERIKEILRE
TGLEPNRLEIEVTENVTLSDPEKTLQTMKSLKKMGVRILIDDFGTGYASLSYLRKFQFDG
LKLDKSFIFTLGDSPQNQSVVEKIIDLGKAYSMAVTAEGVETAEQLSFLKKNKCDEVQGY
LLGKPAAITDLNLNANRVNL