Protein Info for EX28DRAFT_1237 in Enterobacter asburiae PDN3

Annotation: Carboxylesterase type B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00135: COesterase
  • amino acids 6 to 479 (474 residues), 324.9 bits, E=1.8e-100
• PF20434: BD-FAE
  • amino acids 89 to 199 (111 residues), 37.9 bits, E=2.2e-13

Best Hits

KEGG orthology group: K03927, carboxylesterase type B [EC: 3.1.1.1] (inferred from 91% identity to enc:ECL_02040)

Predicted SEED Role

"Putative esterase"

MetaCyc Pathways

• methylsalicylate degradation (2/2 steps found)
• aspartame degradation (1/2 steps found)
• methyl indole-3-acetate interconversion (1/2 steps found)
• superpathway of methylsalicylate metabolism (1/5 steps found)
• retinol biosynthesis (2/7 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis II
• Drug metabolism - other enzymes

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.1

Protein Sequence (501 amino acids)

>EX28DRAFT_1237 Carboxylesterase type B (Enterobacter asburiae PDN3)
MQNPSAPVVETRQGALTGLTDENVHLWCGIPYAAPPVGEWRWRSPRPPERWDGLREATAF
SPSSWQSSEYCQELGGGDPGQFSEDCLYLNVWSPTERAEPLPVMVWLHGGGFTIGAGGLP
PYNGKALATRGVVVVTINYRLGHLGFFAHPALEGEEGRVVHNFALLDQIAALEWVRENIA
GFGGDPANVTLFGESAGARSVLSLLASPLAEGLFHKAIVQSGYTLPDTPREQALQKGEAL
AAHFGLENATAGQLRAIPPEAFWPLTAPLNVAPAPIAGDCVLPEAMLDVFFAARQHPVPV
MIGSNSDEASVMAVFGIDLAGQIQKLRRERRFGLGLIKLLYPGVKGDEELGRQVCRDMAF
TTMGYVVMQAQQRVGGLCWRYWFDYVAEAEHATYINGAWHGNEVPYVFDTLGQVEPSRQY
VNERDLQFAAHVADYWVSFARDAGSRDILPGPTHWPACRKGRDVLLRIGVNKHAAFKLEN
RFMRARMSLFKRVMKHHVSLD