Protein Info for EX28DRAFT_1191 in Enterobacter asburiae PDN3

Annotation: Mannitol-1-phosphate/altronate dehydrogenases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 PF01232: Mannitol_dh" amino acids 16 to 262 (247 residues), 162.1 bits, see alignment E=1.8e-51 PF08125: Mannitol_dh_C" amino acids 273 to 470 (198 residues), 189.2 bits, see alignment E=5.6e-60

Best Hits

Swiss-Prot: 89% identical to UXAB_ENT38: Altronate oxidoreductase (uxaB) from Enterobacter sp. (strain 638)

KEGG orthology group: K00041, tagaturonate reductase [EC: 1.1.1.58] (inferred from 89% identity to ent:Ent638_2013)

MetaCyc: 88% identical to tagaturonate reductase (Escherichia coli K-12 substr. MG1655)
Tagaturonate reductase. [EC: 1.1.1.58]

Predicted SEED Role

"Altronate oxidoreductase (EC 1.1.1.58)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 1.1.1.58)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.58

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (483 amino acids)

>EX28DRAFT_1191 Mannitol-1-phosphate/altronate dehydrogenases (Enterobacter asburiae PDN3)
MNTLNRRDFPGAQYPERIIQFGEGNFLRAFIDWQIDLLNEHTDLNAGVVIVRPIQSDFPP
SLNTQDGLYTTIIRGLNGQDEAVSESRLIRSVNREIDVYSQYDAFLKLAHNPDMRFVFSN
TTEAGISYHAGDRFDDAPAVSYPAKLTRLLFERFSHFNGAADKGWIIIPCELIDYNGDAL
RELVLRYAREWALPAAFIRWLNDANSFCSTLVDRIVTGYPRDEAAMLEAELGYHDGFLDT
AEHFYLFVIQGPASLAQELCLDKYPLNVLIVDDIRPYKERKVAILNGAHTALVPVAFQAG
LDTVGEAMNDAEICAFVEKAIYQEIIPVLDLPRDELESFASAVTGRFRNPYIRHQLLSIA
LSGMTKYRTRILPQLLAGQNASGQLPARLTFALAALIAFYRGERNGENYPVQDDAFWLER
YQQLWAQHRDQQLSTRELVQSVLSVSEHWEQDLTQVSGLVDQVTLDLDAILLKGMRAAVN
PLC