Protein Info for EX28DRAFT_0987 in Enterobacter asburiae PDN3

Annotation: Trehalose and maltose hydrolases (possible phosphorylases)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 759 PF03636: Glyco_hydro_65N" amino acids 11 to 239 (229 residues), 172.3 bits, see alignment E=2.1e-54 PF03632: Glyco_hydro_65m" amino acids 302 to 663 (362 residues), 448.9 bits, see alignment E=2e-138 PF03633: Glyco_hydro_65C" amino acids 673 to 731 (59 residues), 37.3 bits, see alignment 2.8e-13

Best Hits

Swiss-Prot: 63% identical to KOJP_ECOLI: Kojibiose phosphorylase (ycjT) from Escherichia coli (strain K12)

KEGG orthology group: K04844, hypothetical glycosyl hydrolase [EC: 3.2.1.-] (inferred from 85% identity to enc:ECL_01787)

MetaCyc: 63% identical to kojibiose phosphorylase (Escherichia coli K-12 substr. MG1655)
Kojibiose phosphorylase. [EC: 2.4.1.230]

Predicted SEED Role

"Maltose phosphorylase (EC 2.4.1.8) / Trehalose phosphorylase (EC 2.4.1.64)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis or Trehalose Uptake and Utilization (EC 2.4.1.64, EC 2.4.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 2.4.1.230 or 2.4.1.64 or 2.4.1.8 or 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (759 amino acids)

>EX28DRAFT_0987 Trehalose and maltose hydrolases (possible phosphorylases) (Enterobacter asburiae PDN3)
MLNVSVLTDPGFCPHSLNKYASIMACGNGYMGIRAAHEEDYTQQTRGMYLAGLYHQAGRN
ETTELVNLPDIIDIDVELDGVNFTLLSGEILEWQRELAFANGELRRSVVWRSPDGKRYRL
ESRRFVSLDQLPLVAMQLSITPLDAATQAVLKTGIDATQTNSGRQHLDEMSVRVFDQEYM
QGVYETQDRASEVVVSAFCQLSAKSDSCFTAKNRRLSVHHSLTIAMGDTVTLEKIVWLTH
RSDKALSQASFARNALADLKVCAARGYDALLESSACAWQEVWRDARVEVDSTEHQDQIAL
DYAVWHLTTMTPAHDERSSIAAKGLTGEGYKGHVFWDTEIFLLPFHLFTRPQTARSLLRY
RWLNLAGAREKARRNGWPGALFPWESAASGLEETPEFAAINIRTGTRQKVASALAEHHIV
ADIAWAVVAYWQATHDDAFMRNEGLTLLMETAAFWMGRATEINGRLEIHDVIGPDEYTEH
VNNNAYTNYLAWHNVACARQFMAIFGREDEGFTQNATRFLTRLWLPEANPDGVIPQDDTF
MAKPAIDLSRYKAKAGKQTILLDYSRAEVNEMQILKQADVVMLTYLLPERFTPQQCAANL
AFYEPRTIHDSSLSKAIHGIVTARCGDVEGAYAFWRDGVAIDLGDDPHSCDDGIHAAATG
AIWSGVIQGFAGMQIVEGELHLAPKLPAHWQKLTFPLRWRNATLHFTYENEVLTIEASAP
ATLTLWGKTLHLSGRKICAYKDFLASQNGTATTEGGHDA