Protein Info for EX28DRAFT_0980 in Enterobacter asburiae PDN3

Annotation: Glycosidases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 transmembrane" amino acids 406 to 427 (22 residues), see Phobius details PF00128: Alpha-amylase" amino acids 87 to 342 (256 residues), 73.1 bits, see alignment E=1.4e-24

Best Hits

Swiss-Prot: 67% identical to GGAP_ECOLI: Glucosylglycerate phosphorylase (ycjM) from Escherichia coli (strain K12)

KEGG orthology group: K00690, sucrose phosphorylase [EC: 2.4.1.7] (inferred from 82% identity to enc:ECL_01780)

MetaCyc: 67% identical to glucosylglycerate phosphorylase (Escherichia coli K-12 substr. MG1655)
RXN-18999 [EC: 2.4.1.352]

Predicted SEED Role

"Sucrose phosphorylase (EC 2.4.1.7)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Sucrose utilization or Sucrose utilization Shewanella (EC 2.4.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.352 or 2.4.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (565 amino acids)

>EX28DRAFT_0980 Glycosidases (Enterobacter asburiae PDN3)
MENKVNTKIKELINLVYGETFSEAHLNVLLENIGKAASVITEKRKTGWDEKDVVLITYAE
QFSAKGEKALPVFTRFYNRWLSHSFSHVHLLPFYPWSSDDGFSVIDYHEVAPETGTWRDV
AELKQSASLMFDFVCNHMSAKSIWFANYLKQIPGYDDFFISVDPETDLSAVTRPRALPLL
TPFTLHDGSVRHLWTTFSEDQVDLNFASPQVLIAMVDVLLHYLIEGARYIRLDAVGFMWK
LPGTNCIHLEQTHQLIQLFRAITEAVAPGTVIITETNVPHKDNVAYFGDGENEAQMVYQF
SLPPLVLHAVHSQDVRTLSQWAESLTLPSTKTTWFNFLASHDGIGLNPLRGILPESEILS
LVEKLQAEGALVNWKNNPDGTRSPYEINVTYLDALSTRNSPDSQRIARFILAHAVLLSFP
GVPAIYIQSILGSRNDYAGVERLGYNRAINREKYAAGQIDSVLEDKNSLRSQVYCSLSQL
IALRRAGKAFHPDSEAYFSASGEHVLKIVRVANCGEKITALFNFSDGIQTVESDIQSGTE
LISGKDITDKTLTLYPWQVLWIKEN