Protein Info for EX28DRAFT_0785 in Enterobacter asburiae PDN3

Annotation: oxidoreductase alpha (molybdopterin) subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01701: oxidoreductase alpha (molybdopterin) subunit
  • amino acids 18 to 753 (736 residues), 968.8 bits, E=8.7e-296
• PF00384: Molybdopterin
  • amino acids 114 to 482 (369 residues), 80.7 bits, E=1.1e-26
• PF01568: Molydop_binding
  • amino acids 642 to 749 (108 residues), 52.7 bits, E=4.1e-18

Best Hits

Swiss-Prot: 54% identical to YDEP_ECO57: Protein YdeP (ydeP) from Escherichia coli O157:H7

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 92% identity to enc:ECL_01530)

Predicted SEED Role

"Putative formate dehydrogenase oxidoreductase protein" in subsystem Formate hydrogenase

MetaCyc Pathways

• formate oxidation to CO₂ (1/1 steps found)
• oxalate degradation VI (1/4 steps found)
• oxalate degradation III (1/5 steps found)
• purine nucleobases degradation II (anaerobic) (13/24 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Protein Sequence (762 amino acids)

>EX28DRAFT_0785 oxidoreductase alpha (molybdopterin) subunit (Enterobacter asburiae PDN3)
MNKKRRSVPGIRHYDGPAGGWGALKATAIAVRTQMDTLDAPATLLRTNQPDGFDCPGCAW
PDKEHKSTFQFCENGAKAVTWEATSKRLTPEFLAENSVSSLLAKSDFELEGYGRLTHPLR
YDRASDTFRPVDWDEAFQHIGEVLRGLEPDQVEFYTSGRASNEAAYLFQLFAREYGTNNF
PDCSNMCHEATSVGLPRSIGIGKGTVSLDDFDKTELVISIGHNPGTNHPRMMGTLHELAR
RNVPIIVFNPLRERALERFADPQSVIEMATYGSTDIASTYFQVKAGGDAAALKGIAKHLL
EMEAERGDVLDRAFIAEHTQGIEDFAADIAQTRWDEIERESGLNRAALGKVADAYAKSNA
TIITYGMGITQHNKGTANVRLIADLLLLRGNIGKPGAGICPLRGHSNVQGNRTVGISEKP
TPAFLNRLKDVFGFEPPSHHGHDAVQATQAMIDGRAKALICLGGNFAVAMPDHERGFPAM
GNLDLSVHVGTKLNRTHLLVGKETFIFPCLGRTELDLQAAGRQSITVEDSMSMVHASSGK
LKPASPLLLSEPAIVAGMAKATLPETRVDWLTLIEDYDRIRDLIEQTIPGFENYNARIRV
PGGFRMPLPPTQRIWPTATGKAMFSVFDGVHENASGEGEHVLRLITLRSHDQYNTTIYAL
DDRYRGVFGRRDVLFMNEDDMAQSGLEHGDRVDIETALPGSVQRLKDITVVAYNIAPGTV
GAYYPEANVLVPLDYLDKDSGTPSYKSVPVRITLRSKEIRML