Protein Info for EX28DRAFT_0598 in Enterobacter asburiae PDN3

Annotation: diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 602 transmembrane" amino acids 64 to 82 (19 residues), see Phobius details amino acids 397 to 420 (24 residues), see Phobius details PF17151: CHASE7" amino acids 81 to 266 (186 residues), 230.1 bits, see alignment E=1.7e-72 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 434 to 600 (167 residues), 191.6 bits, see alignment E=4.1e-61 PF00990: GGDEF" amino acids 438 to 597 (160 residues), 153 bits, see alignment E=6e-49

Best Hits

Swiss-Prot: 61% identical to DGCQ_ECOUT: Probable diguanylate cyclase DgcQ (dgcQ) from Escherichia coli (strain UTI89 / UPEC)

KEGG orthology group: None (inferred from 82% identity to enc:ECL_03237)

MetaCyc: 60% identical to diguanylate cyclase DgcQ (Escherichia coli K-12 substr. MG1655)
Diguanylate kinase. [EC: 2.7.7.65]

Predicted SEED Role

"FIG00627063: hypothetical protein"

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.65

Use Curated BLAST to search for 2.7.7.65

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (602 amino acids)

>EX28DRAFT_0598 diguanylate cyclase (GGDEF) domain (Enterobacter asburiae PDN3)
MSFSRETGSTSGTRLNHITPGFAGRFYLKYRVKGSDQGVCVQRDTFVVRQSFLQWLHKRS
NPGLIVNLCFLVVLVFSTLLTWREVVVLEEAYISSQRNHLDTVASSLDRQLQNSVDRMLL
FRQGMRDAIQTPLAFEVLQNAVSRFDTVRMLPTWQLAVDKKRTLPINGVSDAFVQKTTLL
NRDDERLSHEISAALEVGYLLRLASSRAQTEARVIYVSRAGFFISTDTPDQAGDITSRYY
HLVTQSWFTQQSERNNRARAVRWFISTPSSWTNDERTITASVPIYFDHYWYGVVAMDFTL
NTMQRLLADATEDRTEGEYQLYDTRLNMIATSAHTGNVVNHFDERETAQIAQAIEHATGG
GIRLGSRFVSWERLDHFDGVVLRIHTLHEGVQDDFGSISIVLALLWALFTAMLLISWLVI
RRMVSNMYTLQHSLQWQAWHDPLTRLNNRGALFDRAKLLAETCRQQSLPFSVIQIDLDHF
KNINDRFGHQAGDKVLSHTAGLIASALRKNDVAGRVGGEEFCVVLPGIGLEEARSVADRI
RCRINSKEILVKKSTTLRISASLGVSSADEAGNYEFEQLQSVADVRLYQAKQSGRNRVVW
RD