Protein Info for EX28DRAFT_0576 in Enterobacter asburiae PDN3

Annotation: Siderophore synthetase component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 580 transmembrane" amino acids 486 to 507 (22 residues), see Phobius details PF04183: IucA_IucC" amino acids 141 to 356 (216 residues), 167.1 bits, see alignment E=5.1e-53 PF06276: FhuF" amino acids 405 to 560 (156 residues), 105 bits, see alignment E=5.9e-34

Best Hits

Swiss-Prot: 66% identical to IUCA_ECOLX: N(2)-citryl-N(6)-acetyl-N(6)-hydroxylysine synthase (iucA) from Escherichia coli

KEGG orthology group: K03894, aerobactin synthetase subunit alpha [EC: 6.3.2.27] (inferred from 92% identity to enc:ECL_03316)

MetaCyc: 66% identical to citrate:N6-acetyl-N6-hydroxy-L-lysine ligase (Escherichia coli K-12)
RXN-13054 [EC: 6.3.2.38]

Predicted SEED Role

"Citrate:6-N-acetyl-6-N-hydroxy-L-lysine ligase, alpha subunit (EC 6.3.2.27), aerobactin biosynthesis protein IucA @ Siderophore synthetase superfamily, group A @ Siderophore synthetase large component, acetyltransferase" (EC 6.3.2.27)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.27

Use Curated BLAST to search for 6.3.2.27 or 6.3.2.38

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (580 amino acids)

>EX28DRAFT_0576 Siderophore synthetase component (Enterobacter asburiae PDN3)
MRALPRSAGTDVAAQCFLNALLRETKDWRYLPATAADALPNIHIPLSQTQALRVPVRYFS
PTQHHQYRFPATLIQSSSDDGDAVTFDQLLDLIIEKPSVKGSLDADTLARFKQRVLESHA
HTWQAIDLRHGWANLRDKPLTFADAEQALLVGHAFHPAPKSHEPFSETEARRYLPDFASR
FPLRWFAVESTLVAGDSLNVSLRERLLRFTAQSAPELLGHFTDTRWLLPMHPWQADYLLE
QDWCQRLAENGSLQDLGEAGAQWLPTSSSRSLYSETNGDMIKFSLSVRLTNSVRTLSVKE
VKRGMRLARLAKTERWQDLQARYPTMRVMQEDGWAGLRDESGIIQEESLMALRVNLLFDT
PETQTNVLVSLTQAAPDGGDSLLASAVRRLSQRLELPLAQAARCWLDAYCDRVLLPLFSA
EADYGLVLLAHQQNILVEMQQDFPVGLIYRDCQGSAWTEGADGWLKEAGETEVENRFGES
QLLRYFPYYLLLNSTLAVTAALAAAGFDSEENLMSRVRDALTELRKTAKQTRCLDYVLDS
PTWNCKGNFFCYLHDRNENTIADPAVIYFDFSNPFYKEKA