Protein Info for EX28DRAFT_0396 in Enterobacter asburiae PDN3

Annotation: PTS system, fructose-specific, IIB component/PTS system, fructose subfamily, IIC component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 561 transmembrane" amino acids 233 to 256 (24 residues), see Phobius details amino acids 264 to 291 (28 residues), see Phobius details amino acids 301 to 327 (27 residues), see Phobius details amino acids 347 to 367 (21 residues), see Phobius details amino acids 379 to 400 (22 residues), see Phobius details amino acids 425 to 449 (25 residues), see Phobius details amino acids 461 to 480 (20 residues), see Phobius details amino acids 486 to 507 (22 residues), see Phobius details amino acids 516 to 547 (32 residues), see Phobius details PF02302: PTS_IIB" amino acids 106 to 191 (86 residues), 74.3 bits, see alignment E=1e-24 TIGR00829: PTS system, Fru family, IIB component" amino acids 106 to 189 (84 residues), 131.9 bits, see alignment E=8.1e-43 TIGR01427: PTS system, Fru family, IIC component" amino acids 213 to 550 (338 residues), 489.2 bits, see alignment E=8.1e-151 PF02378: PTS_EIIC" amino acids 231 to 494 (264 residues), 66.5 bits, see alignment E=2.2e-22

Best Hits

Swiss-Prot: 91% identical to PTFBC_ECOLI: PTS system fructose-specific EIIB'BC component (fruA) from Escherichia coli (strain K12)

KEGG orthology group: K02769, PTS system, fructose-specific IIB component [EC: 2.7.1.69] K02770, PTS system, fructose-specific IIC component (inferred from 97% identity to enc:ECL_03470)

MetaCyc: 91% identical to fructose-specific PTS multiphosphoryl transfer protein FruA (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69)" in subsystem Fructose utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (561 amino acids)

>EX28DRAFT_0396 PTS system, fructose-specific, IIB component/PTS system, fructose subfamily, IIC component (Enterobacter asburiae PDN3)
MKTLLIIDSGLGQARAYMAKTLLGAAAQKAHLDIIDNPGDAELAIVLGDKIPADSALNGK
KVWLGDINRAVAHPELFLSEAKGHASVYSAPVATAPAAAAGPKRIVAVTACPTGVAHTFM
AAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAEADLVIVAADIEVDLAKFAGKPMY
RTSTGLALKKTAQEFDKALVEAKPYQATGARQTATEGKKESAGAYRHLLTGVSYMLPMVV
AGGLCIALSFAFGITAFKEQGTLAAALMQIGGGSAFALMVPVLAGFIAFSIADRPGLTPG
LIGGMLAVSTGSGFIGGIIAGFLAGYVAKLISSKLKLPQSMEALKPILIIPLISSLVVGL
AMIYLIGKPVAGILAGLTHWLQTMGTANAVLLGAILGGMMCTDMGGPVNKAAYAFGVGLL
STQTYAPMAAIMAAGMVPPLALGLATIIARRKFDKAQQEGGKAALVLGLCFITEGAIPFA
ARDPMRVLPCCIAGGAVTGAISMAIGAKLMAPHGGLFVLLIPGAITPVLGYLLAIVAGTL
VAGLSYAVLKRPEAEVVAKAA