Protein Info for EX28DRAFT_0060 in Enterobacter asburiae PDN3

Annotation: metabolite-proton symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 transmembrane" amino acids 34 to 52 (19 residues), see Phobius details amino acids 61 to 83 (23 residues), see Phobius details amino acids 95 to 115 (21 residues), see Phobius details amino acids 121 to 141 (21 residues), see Phobius details amino acids 161 to 182 (22 residues), see Phobius details amino acids 194 to 213 (20 residues), see Phobius details amino acids 243 to 262 (20 residues), see Phobius details amino acids 277 to 299 (23 residues), see Phobius details amino acids 311 to 330 (20 residues), see Phobius details amino acids 336 to 359 (24 residues), see Phobius details amino acids 370 to 392 (23 residues), see Phobius details amino acids 404 to 422 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 23 to 220 (198 residues), 90.6 bits, see alignment E=2.1e-29 amino acids 219 to 420 (202 residues), 47.7 bits, see alignment E=2.2e-16 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 29 to 416 (388 residues), 471.2 bits, see alignment E=1.5e-145 PF07690: MFS_1" amino acids 63 to 381 (319 residues), 91.5 bits, see alignment E=1e-29 amino acids 256 to 421 (166 residues), 42.5 bits, see alignment E=8.4e-15

Best Hits

Swiss-Prot: 85% identical to KGTP_SHIFL: Alpha-ketoglutarate permease (kgtP) from Shigella flexneri

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 96% identity to enc:ECL_03923)

MetaCyc: 85% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"Alpha-ketoglutarate permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (431 amino acids)

>EX28DRAFT_0060 metabolite-proton symporter (Enterobacter asburiae PDN3)
MTESITSNGTLANSDTRRRVWAIVSASSGNLVEWFDFYVYSFCSLYFAHIFFPSGNTTTQ
LLQTAGVFAAGFLMRPIGGWLFGRIADRRGRKASMLISVCMMCFGSLVIACLPGYESIGT
WAPALLLLARLFQGLSVGGEYGTSATYMSEIALEGRKGFYASFQYVTLIGGQLLAILVVV
ILQQILTDEQLHAWGWRIPFAMGAVLAVVALWLRRQLDETSQKEVRTLKEAGTFKGLWRN
RKAFLMVLGFTAGGSLSFYTFTTYMQKYLVNTTGMHANVASVVMTVALFVFMLIQPIIGA
LSDKIGRRTSMLIFGGLSALCTVPILSALQHVTSPYAAFALVMLAMVIVSFYTSISGILK
AEMFPAQVRALGVGLSYAVANALFGGSAEYVALSLKSWGSETTFFWYVTIMGALAFIVSL
MLHRKGKGIRL