Protein Info for EFB2_00485 in Escherichia fergusonii Becca

Annotation: putative bifunctional chitinase/lysozyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 899 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF02839: CBM_5_12" amino acids 463 to 496 (34 residues), 31.5 bits, see alignment (E = 7.7e-12)

Best Hits

Swiss-Prot: 95% identical to CHIA_ECOLI: Probable bifunctional chitinase/lysozyme (chiA) from Escherichia coli (strain K12)

KEGG orthology group: K13381, bifunctional chitinase/lysozyme [EC: 3.2.1.14 3.2.1.17] (inferred from 95% identity to eco:b3338)

MetaCyc: 95% identical to endochitinase (Escherichia coli K-12 substr. MG1655)
Chitinase. [EC: 3.2.1.14]

Predicted SEED Role

"Chitinase (EC 3.2.1.14)" in subsystem Chitin and N-acetylglucosamine utilization (EC 3.2.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.17

Use Curated BLAST to search for 3.2.1.14 or 3.2.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (899 amino acids)

>EFB2_00485 putative bifunctional chitinase/lysozyme (Escherichia fergusonii Becca)
MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGK
AKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSTSNTPASNAPASNTPANGGSA
TPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNPWRY
VRAATATEISETSNPQSCTSAPQPAPDVKPAPDVQPAPADKSNDNYAVVAWKGQEGSSTW
YVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEITQYGNPGSCSVKPDNN
GGAVTPVDPTPETPETPVTPTPDNNEPSTPADSSNDYSLQAWSGQEGSEIYHVIFNGNVY
QNAWWVGSEDCPRGTSVENSNNPWRLVRTATAAEMSQYGNPTTCEIDNGGVIIADGFQAS
KAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWSASTVYVKGDRVVVDG
QAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKTQSYSNEELNNAPQFNPETLYASDT
LIRFNGENYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLN
TIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVM
LSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVK
KVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQ
SANTEGQNIHGKCATSAIANLHAQLKGLFGNKSDAEIDAMMGTTPMVGVNDVQGEVFYLS
DARLVMQDAQKRNLGMVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ