Protein Info for ECD_04087 in Escherichia coli BL21

Annotation: putative transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 185 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01626: conserved hypothetical protein YtfJ-family, TIGR01626" amino acids 1 to 183 (183 residues), 306.7 bits, see alignment E=2.6e-96 PF09695: YtfJ_HI0045" amino acids 24 to 181 (158 residues), 256.4 bits, see alignment E=4.6e-81

Best Hits

Swiss-Prot: 98% identical to YTFJ_ECOLI: Uncharacterized protein YtfJ (ytfJ) from Escherichia coli (strain K12)

KEGG orthology group: K07109, (no description) (inferred from 98% identity to sdy:SDY_4453)

Predicted SEED Role

"Protein ytfJ precursor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (185 amino acids)

>ECD_04087 putative transcriptional regulator (Escherichia coli BL21)
MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDKDQFSYKTWNSAQLVGK
VRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNTDDAIPGSGMFVRSSLESN
KKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQWAKDGALTQEEVQQVMDLLH
KLINK