Protein Info for ECD_04034 in Escherichia coli BL21

Annotation: bifunctional NAD(P)H-hydrate repair enzyme; C-terminal domain ADP-dependent (S)-NAD(P)H-hydrate dehydratase and N-terminal domain NAD(P)H-hydrate epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 515 TIGR00197: YjeF family N-terminal domain" amino acids 17 to 220 (204 residues), 239.2 bits, see alignment E=3.5e-75 PF03853: YjeF_N" amino acids 40 to 199 (160 residues), 151.8 bits, see alignment E=1.9e-48 TIGR00196: YjeF family C-terminal domain" amino acids 231 to 499 (269 residues), 329 bits, see alignment E=2.4e-102 PF01256: Carb_kinase" amino acids 258 to 496 (239 residues), 285.9 bits, see alignment E=2.6e-89

Best Hits

Swiss-Prot: 99% identical to NNR_ECOLI: Bifunctional NAD(P)H-hydrate repair enzyme Nnr (nnr) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ebr:ECB_04034)

MetaCyc: 99% identical to NAD(P)HX epimerase / NAD(P)HX dehydratase (Escherichia coli K-12 substr. MG1655)
RXN-12752 [EC: 5.1.99.6]; 5.1.99.6 [EC: 5.1.99.6]; RXN-13141 [EC: 5.1.99.6, 4.2.1.136]; 4.2.1.136 [EC: 5.1.99.6, 4.2.1.136]

Predicted SEED Role

"NAD(P)HX epimerase / NAD(P)HX dehydratase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.136 or 5.1.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (515 amino acids)

>ECD_04034 bifunctional NAD(P)H-hydrate repair enzyme; C-terminal domain ADP-dependent (S)-NAD(P)H-hydrate dehydratase and N-terminal domain NAD(P)H-hydrate epimerase (Escherichia coli BL21)
MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLRAGEAAFQVCRSAYPDA
RHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLPEEAALAREAWLNAGGEIH
ASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSHPAPIAAVDIPSGLLAETGAT
PGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLGLDSWLAGQETKIQRFSAEQLSH
WLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEAALRAGAGLVRVLTRSENIAPLLTA
RPELMVHELTMDSLAESLEWADVVVIGPGLGQQEWGKKALQKVENFRKPMLWDADALNLL
AINPDKRHNRVITPHPGEAARLLGCSVAEIESDRLHCAKRLVQRYGGVAVLKGAGTVVAA
HPDALGIIDVGNAGMASGGMGDVLSGIIGALLGQKLSPYDAACAGCVAHGAAADVLAARF
GTRGMLATDLFSTLQRIVNPEVTDKNHDESSNPAP