Protein Info for ECD_03763 in Escherichia coli BL21

Annotation: putative sulfolipid alpha-glucosidase; alpha-glucosyl fluoride glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 667 PF13802: Gal_mutarotas_2" amino acids 80 to 151 (72 residues), 32.8 bits, see alignment E=1.4e-11 PF01055: Glyco_hydro_31_2nd" amino acids 256 to 546 (291 residues), 159.1 bits, see alignment E=2.9e-50 PF21365: Glyco_hydro_31_3rd" amino acids 572 to 655 (84 residues), 80 bits, see alignment E=2.3e-26

Best Hits

Swiss-Prot: 100% identical to SQASE_ECOLI: Sulfoquinovosidase (yihQ) from Escherichia coli (strain K12)

KEGG orthology group: K01811, putative family 31 glucosidase (inferred from 100% identity to ebd:ECBD_4149)

MetaCyc: 100% identical to sulfoquinovosidase (Escherichia coli K-12 substr. MG1655)
RXN-17700 [EC: 3.2.1.199]; 3.2.1.199 [EC: 3.2.1.199]

Predicted SEED Role

"Alpha-glucosyltransferase YihQ"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.199

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (667 amino acids)

>ECD_03763 putative sulfolipid alpha-glucosidase; alpha-glucosyl fluoride glucosidase (Escherichia coli BL21)
MRIILHKPVITGRQQRLILTHSKDNPCLWIGSGIADIDMFRGNFSIKDKLQEKIALTDAI
VSQSPDGWLIHFSRGSDISATLNISADDQGRLLLELQNDNLNHNRIWLRLAAQPEDHIYG
CGEQFSYFDLRGKPFPLWTSEQGVGRNKQTYVTWQADCKENAGGDYYWTFFPQPTFVSTQ
KYYCHVDNSCYMNFDFSAPEYHELALWEDKATLRFECADTYISLLEKLTALLGRQPELPD
WIYDGVTLGIQGGTEVCQKKLDTMRNAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSE
NYPQLDSRIKQWNQEGVQFLAYINPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFY
GGVVDLTNPEAYAWFKEVIKKNMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPA
LWAKCNYEALEETGKLSEILFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAAL
SLAMTGHGLHHSDIGGYTTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGD
AETIAHFARMTTVFTTLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGR
DILVAPVHEEGRSDWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAAL
FASLKSI