Protein Info for ECD_03549 in Escherichia coli BL21

Annotation: cryptic adenine deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01178: adenine deaminase
  • amino acids 31 to 584 (554 residues), 840.6 bits, E=2.6e-257
• PF01979: Amidohydro_1
  • amino acids 82 to 362 (281 residues), 134.7 bits, E=7.4e-43
• PF13382: Adenine_deam_C
  • amino acids 434 to 579 (146 residues), 185.3 bits, E=9.7e-59

Best Hits

Swiss-Prot: 100% identical to ADEC_ECODH: Adenine deaminase (ade) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K01486, adenine deaminase [EC: 3.5.4.2] (inferred from 100% identity to eco:b3665)

MetaCyc: 100% identical to adenine deaminase (Escherichia coli K-12 substr. MG1655)
Adenine deaminase. [EC: 3.5.4.2]; Catalase. [EC: 3.5.4.2, 1.11.1.21, 1.11.1.6]

Predicted SEED Role

"Adenine deaminase (EC 3.5.4.2)" in subsystem Purine conversions (EC 3.5.4.2)

MetaCyc Pathways

• reactive oxygen species degradation (4/4 steps found)
• adenine salvage (3/3 steps found)
• superoxide radicals degradation (2/2 steps found)
• ethanol degradation IV (2/3 steps found)
• methanol oxidation to formaldehyde IV (1/2 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Purine metabolism
• Tryptophan metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.11.1.6

Use Curated BLAST to search for 1.11.1.21 or 1.11.1.6 or 3.5.4.2

Protein Sequence (588 amino acids)

>ECD_03549 cryptic adenine deaminase (Escherichia coli BL21)
MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIA
GVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPH
EIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQV
TGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIENCHESYQL
EEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEGHIDAL
IRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKVTVQQVLVK
GEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRYRVIDVIHNELIT
HSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLNEGALAATVSHDSHNIVV
IGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTAQSLAEQIDALKAA
ARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFTTLEVTE