Protein Info for ECD_03271 in Escherichia coli BL21

Annotation: RNA 3'-terminal phosphate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 TIGR03399: RNA 3'-phosphate cyclase" amino acids 5 to 324 (320 residues), 401.7 bits, see alignment E=1.2e-124 PF01137: RTC" amino acids 12 to 325 (314 residues), 273.9 bits, see alignment E=7.8e-86 PF05189: RTC_insert" amino acids 184 to 274 (91 residues), 48.4 bits, see alignment E=1.1e-16

Best Hits

Swiss-Prot: 100% identical to RTCA_ECOLC: RNA 3'-terminal phosphate cyclase (rtcA) from Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)

KEGG orthology group: K01974, RNA 3'-terminal phosphate cyclase [EC: 6.5.1.4] (inferred from 100% identity to ebr:ECB_03271)

MetaCyc: 99% identical to RNA 3'-terminal phosphate cyclase (Escherichia coli K-12 substr. MG1655)
RNA-3'-phosphate cyclase. [EC: 6.5.1.4]; 2.7.7.- [EC: 6.5.1.4]; 2.7.7.- [EC: 6.5.1.4]

Predicted SEED Role

"RNA 3'-terminal phosphate cyclase (EC 6.5.1.4)" in subsystem RNA 3'-terminal phosphate cyclase (EC 6.5.1.4)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (338 amino acids)

>ECD_03271 RNA 3'-terminal phosphate cyclase (Escherichia coli BL21)
MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAKPGLLRQHLTAVKAAAE
ICRATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVLQTVLPALWFADGPSRVEV
SGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFYPAGGGVVATEVSPVASFNTL
QLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSLHEQNIHNLPRDQGPGNTVSLEV
ESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLASTAAVGEYLADQLVLPMALAGAGEF
TVAHPSCHLLTNIAVVERFLPVRFSLIETDGVTRVSIE