Protein Info for ECD_03176 in Escherichia coli BL21

Annotation: general secretory pathway component, cryptic

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF21305: type_II_gspD_N0" amino acids 31 to 100 (70 residues), 86.7 bits, see alignment E=1.1e-28 PF03958: Secretin_N" amino acids 127 to 186 (60 residues), 54.2 bits, see alignment E=2.1e-18 PF00263: Secretin" amino acids 195 to 283 (89 residues), 95.1 bits, see alignment E=5.7e-31

Best Hits

KEGG orthology group: K02453, general secretion pathway protein D (inferred from 100% identity to ebd:ECBD_0426)

Predicted SEED Role

"General secretion pathway protein D"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (338 amino acids)

>ECD_03176 general secretory pathway component, cryptic (Escherichia coli BL21)
MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIVGQHLGKTILIDPSVQG
TISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVRSANVKTSPGMIADSSRPG
VGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYEPSNVLILTGRASTINKLIEV
IKRVDVIGTEKQQIIHLEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDF
SKEQVSKVPLLGDIPLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYR
QEQQQRIDGKSKALVGSEDLPVLDENTFNSHAPAPSSR