Protein Info for ECD_03128 in Escherichia coli BL21

Annotation: putative amino acid ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 transmembrane" amino acids 28 to 48 (21 residues), see Phobius details amino acids 98 to 118 (21 residues), see Phobius details amino acids 126 to 147 (22 residues), see Phobius details amino acids 159 to 182 (24 residues), see Phobius details amino acids 194 to 219 (26 residues), see Phobius details amino acids 232 to 250 (19 residues), see Phobius details amino acids 291 to 314 (24 residues), see Phobius details amino acids 334 to 351 (18 residues), see Phobius details TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 156 to 252 (97 residues), 69.6 bits, see alignment E=1.4e-23 PF00528: BPD_transp_1" amino acids 174 to 360 (187 residues), 87.2 bits, see alignment E=6e-29

Best Hits

Swiss-Prot: 100% identical to YHDY_ECOLI: Inner membrane amino-acid ABC transporter permease protein YhdY (yhdY) from Escherichia coli (strain K12)

KEGG orthology group: K09971, general L-amino acid transport system permease protein (inferred from 100% identity to ebr:ECB_03128)

Predicted SEED Role

"Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4)" (TC 3.A.1.3.4)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (367 amino acids)

>ECD_03128 putative amino acid ABC transporter permease (Escherichia coli BL21)
MTKVLLSHPPRPASHNSNRAMVWVRKNLFSSWSNSLLTIGCIWLMWELIPPLLNWAFLQA
NWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINLALLIGLVSIAPMFWKILP
HRGRYIAAWAVIYPLIVWWLMYGGFFALERVETRQWGGLTLTLIIASVGIAGALPWGILL
ALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVMLPLFMAEGTSIDKLIRALVGVIL
FQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQGLVILPQALKLVIPGLVNTIIALF
KDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEGYVFAALIYWIFCFSMSRYSQHLEKRF
NTGRTPH