Protein Info for ECD_02133 in Escherichia coli BL21

Annotation: nitrate reductase, periplasmic, large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 828 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details TIGR01706: periplasmic nitrate reductase, large subunit" amino acids 2 to 828 (827 residues), 1653.3 bits, see alignment E=0 TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 3 to 31 (29 residues), 20.1 bits, see alignment (E = 5.9e-08) PF04879: Molybdop_Fe4S4" amino acids 40 to 92 (53 residues), 71 bits, see alignment 9.5e-24 PF00384: Molybdopterin" amino acids 96 to 567 (472 residues), 244 bits, see alignment E=3.8e-76 PF01568: Molydop_binding" amino acids 714 to 822 (109 residues), 85.4 bits, see alignment E=4.2e-28

Best Hits

Swiss-Prot: 100% identical to NAPA_ECO55: Periplasmic nitrate reductase (napA) from Escherichia coli (strain 55989 / EAEC)

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 100% identity to ebr:ECB_02133)

MetaCyc: 100% identical to periplasmic nitrate reductase subunit NapA (Escherichia coli K-12 substr. MG1655)
Nitrate reductase (cytochrome). [EC: 1.9.6.1]

Predicted SEED Role

"Periplasmic nitrate reductase precursor (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4 or 1.9.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (828 amino acids)

>ECD_02133 nitrate reductase, periplasmic, large subunit (Escherichia coli BL21)
MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKAPCRFCGTGCGVLVGTQ
QGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLLRMKNGKYDKEGEFTPITW
DQAFDVMEEKFKTALKEKGTESIGMFGSGQWTIWEGYAASKLFKAGFRSNNIDPNARHCM
ASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVA
VLSTYQHRSFELADNGIIFTPQSDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIG
YGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYA
DPNKKVISYWTMGFNQHTRGVWANNLVYNLHLLTGKISQPGCGPFSLTGQPSACGTAREV
GTFAHRLPADMVVTNEKHRDICEKKWNIPSGTIPAKIGLHAVAQDRALKDGKLNVYWTMC
TNNMQAGPNINEERMPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAER
RTQFWRQQVQAPGEAKSDLWQLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPE
VSKFPVSELAEDQLNDESRELGFYLQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPV
VNGKETQWRYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGR
VLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETR
GRNRPPQGLVYMPFFDAAQLVNKLTLDATDPLSKETDFKKCAVKLEKV