Protein Info for ECD_02030 in Escherichia coli BL21

Annotation: putative family 25 glycosyl hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 272 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF01183: Glyco_hydro_25" amino acids 70 to 245 (176 residues), 180.3 bits, see alignment E=2.3e-57

Best Hits

Swiss-Prot: 98% identical to YEGX_ECOLI: Uncharacterized protein YegX (yegX) from Escherichia coli (strain K12)

KEGG orthology group: K07273, lysozyme (inferred from 100% identity to ebd:ECBD_1555)

Predicted SEED Role

"Glycosyl hydrolase YegX, family 25"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (272 amino acids)

>ECD_02030 putative family 25 glycosyl hydrolase (Escherichia coli BL21)
MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITDYIHFYGYRPVKSFAIR
IPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKATEGEKLEDPYFSRNWQLSR
ENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVLDVEERGKLSAKELRKRVSQW
LKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAHYYQRRPDNDGMAWRFWQHSDRG
QVDGINGPVDFNVFNGTVEELQAFVDGIKETP