Protein Info for ECD_01982 in Escherichia coli BL21

Annotation: putative citrate/iron-citrate/zinc-citrate efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 45 to 68 (24 residues), see Phobius details amino acids 76 to 96 (21 residues), see Phobius details amino acids 102 to 123 (22 residues), see Phobius details amino acids 133 to 157 (25 residues), see Phobius details amino acids 163 to 185 (23 residues), see Phobius details amino acids 197 to 214 (18 residues), see Phobius details amino acids 222 to 243 (22 residues), see Phobius details amino acids 263 to 282 (20 residues), see Phobius details amino acids 287 to 308 (22 residues), see Phobius details amino acids 329 to 356 (28 residues), see Phobius details amino acids 393 to 418 (26 residues), see Phobius details amino acids 431 to 451 (21 residues), see Phobius details PF07690: MFS_1" amino acids 15 to 405 (391 residues), 173.5 bits, see alignment E=6e-55 amino acids 321 to 459 (139 residues), 40.3 bits, see alignment E=2e-14 TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 16 to 413 (398 residues), 236.1 bits, see alignment E=3.8e-74 PF05977: MFS_3" amino acids 22 to 171 (150 residues), 32.9 bits, see alignment E=2.6e-12

Best Hits

Swiss-Prot: 100% identical to MDTD_ECOLC: Putative multidrug resistance protein MdtD (mdtD) from Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)

KEGG orthology group: None (inferred from 100% identity to eco:b2077)

Predicted SEED Role

"Multidrug transporter MdtD"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (471 amino acids)

>ECD_01982 putative citrate/iron-citrate/zinc-citrate efflux transporter (Escherichia coli BL21)
MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESPLHMHMVIVSYVLTVAV
MLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELLLARALQGVGGAMMVPVGR
LTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEYASWHWIFLINIPVGIIGAIA
TLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSKGTGLSPLTIAGLVAVGVVALVL
YLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIGSGMLPFMTPVFLQIGLGFSPFHAG
LMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATTLGLSLVTLLFMTTALLGWYYVLPFVL
FLQGMVNSTRFSSMNTLTLKDLPDNLASSGNSLLSMIMQLSMSIGVTIAGLLLGLFGSQH
VSVDSGTTQTVFMYTWLSMALIIALPAFIFARVPNDTHQNVAISRRKRSAQ